Pharmacological assessment of the contribution of the arterial baroreflex to sympathetic discharge patterns in healthy humans.
Pharmacological assessment of the contribution of the arterial baroreflex to sympathetic discharge patterns in healthy humans.
复制标题
健康人动脉压力反射对交感神经放电模式贡献的药理学评估。
DOI：
10.1152/jn.00935.2017
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发表时间：
2018
影响因子：
2.5
通讯作者：
Shoemaker,JKevin
中科院分区：
文献类型：
--
作者：
Limberg,JacquelineK;Ott,ElizabethP;Holbein,WalterW;Baker,SarahE;Curry,TimothyB;Nicholson,WayneT;Joyner,MichaelJ;Shoemaker,JKevin
为了研究压力感受器传入活动的变化如何影响交感神经激活模式，我们通过静脉注射硝普钠 (NTP) 和去氧肾上腺素 (PE) 来控制动脉血压，并使用基于小波的方法测量动作电位 (AP) 模式。我们假设 1) 压力反射卸载 (NTP) 会增加低阈值轴突的放电和潜在轴突的募集，2) 压力反射负载 (PE) 会减少低阈值轴突的放电。在基线和稳态全身静脉内 NTP (0.5–1.2 µg·kg−1·min−1,n= 13) 或 PE (0.2–1.0 µg·kg−1·min−1,n= 9) 输注期间测量心率（HR、ECG）、动脉血压（BP、臂导管）和肌肉交感神经活动（MSNA，腓神经显微神经造影）。随着 NTP 的进行，血压降低，HR 和综合 MSNA 增加（P<0.01）。 AP发生率（326 ± 66至579 ± 129 AP/100次心跳）和每次整合爆发的AP含量（8 ± 1至11 ± 2 AP/爆发）随着NTP的增加而增加（P<0.05）。低阈值轴突的放电概率随着 NTP 的增加而增加，并且观察到高阈值轴突的募集（最大簇数为 22±±3 至 24±±3，每爆发 9±±1 至 11±±1 个簇；P< 0.05）。随着PE的进行，血压升高，HR和综合MSNA降低（P<0.05）。 PE降低AP发生率（406±±128至166±±42 AP/100次心跳），并导致独特簇数减少（最大簇数15±±2至9±±1，P<0.05）；集成突发的组成部分（每个突发的 AP 或簇）没有改变 (P> 0.05)。这些数据支持在操纵压力感受器传入活动期间交感神经激活的分层模式，其中活跃神经元的速率编码起主导作用，以及在稳态低血压应激下招募/取消招募较高阈值单位。新的和值得注意的为了研究压力感受器传入活动的变化如何影响交感神经激活模式，我们用静脉注射硝普钠和去氧肾上腺素操纵动脉血压并测量使用基于小波的方法进行交感神经流出。压力感受反射卸载通过增加低阈值轴突（速率编码）的放电概率和招募新的高阈值轴突群体来增加交感神经活动。压力感受反射负荷通过降低较大轴突的放电概率（征退）来降低交感神经活动；然而，综合爆发的组成部分不受影响。
To study how changes in baroreceptor afferent activity affect patterns of sympathetic neural activation, we manipulated arterial blood pressure with intravenous nitroprusside (NTP) and phenylephrine (PE) and measured action potential (AP) patterns with wavelet-based methodology. We hypothesized that1) baroreflex unloading (NTP) would increase firing of low-threshold axons and recruitment of latent axons and2) baroreflex loading (PE) would decrease firing of low-threshold axons. Heart rate (HR, ECG), arterial blood pressure (BP, brachial catheter), and muscle sympathetic nerve activity (MSNA, microneurography of peroneal nerve) were measured at baseline and during steady-state systemic, intravenous NTP (0.5–1.2 µg·kg−1·min−1,n= 13) or PE (0.2–1.0 µg·kg−1·min−1,n= 9) infusion. BP decreased and HR and integrated MSNA increased with NTP (P< 0.01). AP incidence (326 ± 66 to 579 ± 129 APs/100 heartbeats) and AP content per integrated burst (8 ± 1 to 11 ± 2 APs/burst) increased with NTP (P< 0.05). The firing probability of low-threshold axons increased with NTP, and recruitment of high-threshold axons was observed (22 ± 3 to 24 ± 3 max cluster number, 9 ± 1 to 11 ± 1 clusters/burst;P< 0.05). BP increased and HR and integrated MSNA decreased with PE (P< 0.05). PE decreased AP incidence (406 ± 128 to 166 ± 42 APs/100 heartbeats) and resulted in fewer unique clusters (15 ± 2 to 9 ± 1 max cluster number,P< 0.05); components of an integrated burst (APs or clusters per burst) were not altered (P> 0.05). These data support a hierarchical pattern of sympathetic neural activation during manipulation of baroreceptor afferent activity, with rate coding of active neurons playing the predominant role and recruitment/derecruitment of higher-threshold units occurring with steady-state hypotensive stress.NEW & NOTEWORTHYTo study how changes in baroreceptor afferent activity affect patterns of sympathetic neural activation, we manipulated arterial blood pressure with intravenous nitroprusside and phenylephrine and measured sympathetic outflow with wavelet-based methodology. Baroreflex unloading increased sympathetic activity by increasing firing probability of low-threshold axons (rate coding) and recruiting new populations of high-threshold axons. Baroreflex loading decreased sympathetic activity by decreasing the firing probability of larger axons (derecruitment); however, the components of an integrated burst were unaffected.
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影响因子：
5.5
作者：
Steinback, Craig D.;Salmanpour, Aryan;Shoemaker, J. Kevin
通讯作者：
Shoemaker, J. Kevin
下行抑制通路参与压力感受器反射的证据
DOI：
10.1113/jphysiol.1974.sp010667
发表时间：
1974
期刊：
The Journal of Physiology
影响因子：
--
作者：
J. Coote;V. H. Macleod
通讯作者：
V. H. Macleod
影响因子：
4.9
作者：
Grassi, G;Seravalle, G;Mancia, G
通讯作者：
Mancia, G
影响因子：
3
作者：
Salmanpour, Aryan;Brown, Lyndon J.;Shoemaker, J. Kevin
通讯作者：
Shoemaker, J. Kevin
DOI：
10.1152/ajpheart.00334.2016
发表时间：
2016-10-01
影响因子：
4.8
作者：
Badrov, Mark B.;Lalande, Sophie;Shoemaker, J. Kevin
通讯作者：
Shoemaker, J. Kevin