Regulation by light and darkness of melatonin secretion from the superfused masu salmon (Oncorhynchus masou) pineal organ
Regulation by light and darkness of melatonin secretion from the superfused masu salmon (Oncorhynchus masou) pineal organ
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DOI：
10.1076/brhm.29.1.86.3042
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发表时间：
1998-02-01
影响因子：
1.1
通讯作者：
Aida, K
中科院分区：
文献类型：
--
作者：
Iigo, M;Kitamura, S;Aida, K
研究表明，在人工饲养条件下，大麻哈鱼松果体的形态结构发生了变化。在15 ℃下，在12：12小时（LD 12：12）的光-暗循环或相同的光-暗循环与恒定黑暗（DD）组合72小时下，测定全器官培养（灌流）系统和褪黑激素分泌概况。在LD为12：12的条件下，灌流的松果体在暗相和亮相表现出高速率和低速率的节律性褪黑素分泌。分别当松果体在LD 12：12下维持24 h后，将其转移到DD。褪黑激素分泌被持续激活，没有明显的内源性成分。当维持在DD下48小时的松果体器官被转移到LD 12：12时，褪黑激素分泌仅在光相期间减少。这些结果表明，褪黑激素分泌从灌注的松果体器官的马苏鲑鱼的调节，而不是由内松果体昼夜节律振荡器，但由环境LD周期，通过当地的光感受器。
The pineal organ of masu salmon Oncorhynchus masou was maintained in a. now-through, whole-organ culture (superfusion) system and melatonin secretory profiles were determined at 15 degrees C under light-dark cycles of 12:12 h (LD 12:12) or the same in combination with constant darkness (DD) for 72 h. Under LD 12:12, superfused pineal organs showed a rhythmic melatonin secretion with high and low rates during the dark phase and the light phase. respectively. When the pineal organs maintained under LD 12:12 for 24 h were transferred to DD. melatonin secretion was consistently activated and no endogenous component was evident. When the pineal organs maintained under DD for 48 h were transferred to LD 12:12, melatonin secretion was reduced only during the light phase. These results indicate that melatonin secretion from the superfused pineal organ of masu salmon is regulated not by an intra-pineal circadian oscillator but by the environmental LD cycles, via local photoreceptors.