Blocking antibodies inhibit complete African swine fever virus neutralization
Blocking antibodies inhibit complete African swine fever virus neutralization
复制标题
DOI：
10.1016/s0168-1702(97)01463-9
复制
发表时间：
1997-06-01
期刊：
影响因子：
5
通讯作者：
Escribano, JM
中科院分区：
文献类型：
--
作者：
GomezPuertas, P;Escribano, JM
在体外中和试验中，在大多数恢复期猪血清中发现了持久的非中和非洲猪瘟病毒(ASFV)组分。为了研究这一现象，使用了不同病毒分离株感染的恢复期猪的抗血清，这些抗血清表现出完全或不完全的病毒中和。不同的实验确定ASFV的不完全中和既不是由病毒聚集引起的，也不是由于病毒-抗体复合体的低亲和力或稳定性引起的。此外，从持久组分中提纯抗原性逃逸突变病毒的尝试也没有成功。然而，血清之间的竞争实验表明，血清中存在的抗体具有持久性，抑制了血清中存在的抗体所介导的完全中和，这些抗体中和了100%的病毒感染性。这些结果表明，在ASFV感染过程中诱导封闭抗体可能是中和试验中观察到的持续存活病毒组分的主要原因，也可以解释在一些恢复期猪中观察到的持续感染。(C)1997年爱思唯尔科学公司。
A persistent non-neutralized African swine fever virus (ASFV) fraction is found with most convalescent swine sera in in vitro neutralization assays. To study this phenomenon, antisera from convalescent pigs infected with different virus isolates and showing complete or incomplete virus neutralization were used. Different experiments determined that incomplete neutralization of ASFV is caused neither by virus aggregation, nor low affinity or stability of virus-antibody complexes. Additionally, attempts to purify antigenic escape mutant viruses from the persistent fraction was also unsuccessful. Nevertheless, competition experiments between sera demonstrated that antibodies present in sera showing persistent fraction inhibited the complete neutralization mediated by antibodies present in sera which neutralize 100% of virus infectivity. These results suggest that induction of blocking antibodies during ASFV infection could represent the main cause for the persistent surviving virus fraction observed in neutralization assays and could also explain the persistent infections observed in some convalescent pigs. (C) 1997 Elsevier Science B.V.