Genome-Wide Association Mapping of Seed Coat Color in Brassica napus
Genome-Wide Association Mapping of Seed Coat Color in Brassica napus
复制标题
甘蓝型油菜种皮颜色的全基因组关联图谱
DOI：
10.1021/acs.jafc.7b01226
复制
发表时间：
2017
影响因子：
6.1
通讯作者：
Liu Liezhao
中科院分区：
文献类型：
--
作者：
Wang Jia;Xian Xiaohua;Xu Xinfu;Qu Cunmin;Lu Kun;Li Jiana;Liu Liezhao
Seed coat color is an extremely important breeding characteristic ofBrassica napus. To elucidate the factors affecting the genetic architecture of seed coat color, a genome-wide association study (GWAS) of seed coat color was conducted with a diversity panel comprising 520B. napuscultivars and inbred lines. In total, 22 single-nucleotide polymorphisms (SNPs) distributed on 7 chromosomes were found to be associated with seed coat color. The most significant SNPs were found in 2014 near Bn-scaff_15763_1-p233999, only 43.42 kb away from BnaC06g17050D, which is orthologous toArabidopsis thalianaTRANSPARENT TESTA 12 (TT12), an important gene involved in the transportation of proanthocyanidin precursors into the vacuole. Two of eight repeatedly detected SNPs can be identified and digested by restriction enzymes. Candidate gene mining revealed that the relevant regions of significant SNP loci on the A09 and C08 chromosomes are highly homologous. Moreover, a comparison of the GWAS results to those of previous quantitative trait locus (QTL) studies showed that 11 SNPs were located in the confidence intervals of the QTLs identified in previous studies based on linkage analyses or association mapping. Our results provide insights into the genetic basis of seed coat color inB. napus, and the beneficial allele, SNP information, and candidate genes should be useful for selecting yellow seeds inB. napusbreeding.
