Bone marrow innervation regulates cellular retention in the murine haemopoietic system
Bone marrow innervation regulates cellular retention in the murine haemopoietic system
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DOI：
10.1046/j.1365-2141.1997.2733092.x
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发表时间：
1997-09-01
影响因子：
6.5
通讯作者：
Miyan, JA
中科院分区：
文献类型：
--
作者：
Afan, AM;Boome, CS;Miyan, JA
对小鼠股骨神经支配的解剖分析揭示了造血细胞和基质细胞与神经纤维的密切联系。这些股骨的机械去神经支配导致细胞在24小时内明显动员到外周血中。股细胞数量减少，对动员细胞类型的分析也显示外周血中祖细胞数量增加，在未切除脾的小鼠中，这些祖细胞很快从循环中清除。用6-羟多巴胺对小鼠进行化学交感神经切除术导致骨髓细胞数量减少，但骨髓或外周血祖细胞数量没有变化，也没有观察到全神经去神经后外周血白细胞持续增加。这些观察结果证明了神经系统对动员的选择性控制，也表明了骨髓内增殖的可能控制。我们得出结论，神经支配在维持血液-骨髓界面，控制外周血细胞数量和动员集落形成细胞进入外周血中具有重要作用。
An anatomical analysis of the innervation of murine femora revealed intimate association of haemopoietic and stromal cells with nerve fibres. The mechanical denervation of these femora resulted in significant mobilization of cells into the peripheral blood within 24 h. There was a decrease in femoral cellularity and analysis of the type of cells mobilized also revealed that there was an increase in progenitor cells in the peripheral blood, In non-splenectomized mice these progenitor cells were quickly cleared from the circulation. Chemical sympathectomy of mice with 6-hydroxydopamine resulted in decreased bone marrow cellularity without a change in bone marrow or peripheral blood progenitor cell numbers, nor the sustained rise in peripheral leucocytes observed with whole nerve denervation. These observations argue for selective control of mobilization by the nervous system and also indicate possible control of proliferation within the bone marrow. We conclude that the innervation has an important role in the maintenance of the blood-marrow interface, control of peripheral blood cell numbers, and mobilization of colony forming cells into the periphery.