Virus-Derived Gene Expression and RNA Interference Vector for Grapevine
Virus-Derived Gene Expression and RNA Interference Vector for Grapevine
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DOI：
10.1128/jvi.00436-12
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发表时间：
2012-06-01
影响因子：
5.4
通讯作者：
Dolja, Valerian V.
中科院分区：
文献类型：
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作者：
Kurth, Elizabeth G.;Peremyslov, Valera V.;Dolja, Valerian V.
葡萄树（Vitis vinifera）的农业和酿酒品质的提高受到对传统品种的坚持、这种植物对基因改造的抵制以及公众对转基因生物（GMO）技术的抵制的阻碍。为了应对这些挑战，我们开发了一种基于 RNA 病毒的载体，用于将所需的性状引入葡萄藤中，而无需对基因组进行遗传性修改。该载体在韧皮部组织中表达重组蛋白，参与整个植物（从叶到根再到浆果）的糖运输。此外，该载体还提供强大的RNA干扰（RNAi）能力，通过病毒诱导的基因沉默（VIGS）技术调节内源基因的表达。该载体的其他优点包括卓越的遗传能力和稳定性，以及技术实施的速度。病毒载体最重要的应用包括葡萄树的功能基因组学和通过 RNAi 疫苗接种来对抗病原体或无脊椎害虫进行疾病控制。
The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.