Formation of a cyclic derivative of ethacrynic acid with diazomethane.
Formation of a cyclic derivative of ethacrynic acid with diazomethane.
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与重氮甲烷形成依他尼酸的环状衍生物。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
H. Jun
中科院分区：
文献类型：
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作者：
K. Midha;J. Hubbard;C. Charette;H. Jun
用甲醇-盐酸或重氮甲烷处理依他尼酸样品。GLC和质谱分析表明，甲醇-盐酸反应得到预期的甲酯，而重氮甲烷处理得到含有额外14个质量单位的化合物。精确的质量测量和PMR和IR光谱表明，该产物是依他尼酸甲酯的环状衍生物，4-（2，3-二氢-4-乙基-5-呋喃基）-2，3-二氯苯氧基乙酸甲酯。任一衍生化方法均可用于开发依他尼酸的含量测定。
Samples of ethacrynic acid were treated with methanol-hydrochloric acid or with diazomethane. GLC and mass spectrometric analysis indicated that the methanol-hydrochloric acid reaction gave the expected methyl ester, whereas diazomethane treatment gave a compound containing an additional 14 mass units. Accurate mass measurement and PMR and IR spectra showed that this product was a cyclic derivative of the methyl ester of ethacrynic acid, methyl 4-(2,3-dihydro-4-ethyl-5-furyl)-2,3-dichlorophenoxyacetate. Either derivatization method can be used for development of an assay for ethacrynic acid.