Rat intestinal 25-hydroxyvitamin D3- and 1alpha,25-dihydroxyvitamin D3-24-hydroxylase.
Rat intestinal 25-hydroxyvitamin D3- and 1alpha,25-dihydroxyvitamin D3-24-hydroxylase.
复制标题
大鼠肠道 25-羟基维生素 D3- 和 1α,25-二羟基维生素 D3-24-羟化酶。
DOI：
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发表时间：
1978
影响因子：
4.8
通讯作者：
H. F. Deluca
中科院分区：
文献类型：
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作者：
Rajiv Kumar;H. Schnoes;H. F. Deluca
25-羟基维生素D3和1 α，25-二羟基维生素D3的24-羟基化在体外大鼠肠匀浆中发生。将1 α，24，25-三羟基维生素D3与大鼠肠匀浆一起孵育，分离得到1 α，24，25-三羟基维生素D3，然后通过在高压液相色谱系统上对生物产生的物质与合成的1 α，24 R，25-三羟基维生素D3进行共色谱，并通过紫外光谱、高碘酸盐裂解和质谱进行鉴定。通过在高压液相色谱系统上对生物产生的材料与真实的24 R，25-二羟基维生素D3进行共色谱并通过高碘酸盐裂解来鉴定通过将25-羟基维生素D3与肠匀浆孵育获得的24，25-二羟基维生素D3。组织分布研究表明，该过程发生在大鼠肠匀浆，但不是在大鼠肌肉，肝脏，或胎儿颅骨匀浆。肾切除术不会减少体内1 α，24，25-三羟基维生素D3的产生，但会显著减少，但不会消除24，25-二羟基维生素D3的产生。
The 24-hydroxylation of 25-hydroxyvitamin D3 and 1alpha,25-dihydroxyvitamin D3 occurs in vitro in rat intestinal homogenates. 1alpha,24,25-Trihydroxyvitamin D3 obtained by incubating 1alpha,25-dihydroxyvitamin D3 with rat intestinal homogenates was isolated and then identified by co-chromatography of the biologically generated material with synthetic 1alpha,24R,25-trihydroxyvitamin D3 on a high pressure liquid chromatography system and by ultraviolet spectroscopy, periodate cleavage, and mass spectroscopy. 24,25-Dihydroxyvitamin D3 obtained by incubating 25-hydroxyvitamin D3 with intestinal homogenates was identified by co-chromatography of the biologically generated material with authentic 24R,25-dihydroxyvitamin D3 on a high pressure liquid chromatography system and by periodate cleavage. Tissue distribution studies indicate that the process occurs in rat intestinal homogenates but not in rat muscle, liver, or fetal calvaria homogenates. Nephrectomy does not diminish the production of 1alpha,24,25-trihydroxyvitamin D3 in vivo while it reduces markedly, but does not eliminate, production of 24,25-dihydroxyvitamin D3.