An in vitro study on the efficacy of nanoparticles and nanocomposites as coating materials on surgical sutures
An in vitro study on the efficacy of nanoparticles and nanocomposites as coating materials on surgical sutures
复制标题
关于纳米粒子和纳米复合材料作为手术缝合线涂层材料功效的体外研究
DOI：
10.1038/s41598-025-07558-6
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发表时间：
2025-07-01
影响因子：
3.900
通讯作者：
Omnia M. Abdallah
中科院分区：
文献类型：
--
作者：
Heba R. Shebl;Rehab A. Soliman;Omnia M. Abdallah
关键词：
升级作为主要伤口闭合器械的外科缝线至关重要。细菌耐药性的演变和抗生素的减少已经将研究导向了增强缝线。纳米技术为这些问题提供了答案。除了使用壳聚糖和聚乙烯醇聚合物作为载体之外，通过一锅异位方法使用细菌分离物作为合成银纳米颗粒（AgNP）和银纳米复合材料的生物工厂提供了环境友好的银纳米复合材料。透射电子显微镜（TEM）和zeta电位分析显示球形带负电荷的AgNPs。这些纳米颗粒和纳米复合材料被用作可吸收薇乔和不可吸收真丝外科缝线的涂层。这些涂层缝线的原子力显微镜（AFM）3D图像显示表面粗糙度显著降低，表面形貌改善，特别是壳聚糖-银（CS-Ag）薇乔涂层缝线，纳米复合材料和纳米颗粒薄膜有效附着在缝线表面。场发射扫描电子显微镜和能量色散X射线光谱（FE-SEM/EDX）分析显示，缝线表面上存在大量涂层材料薄膜，并存在大量Ag元素。Vicryl和丝涂层CS-Ag缝线对革兰氏阳性和革兰氏阴性细菌分离株均表现出显著的抗菌和抗菌膜活性。AgNPs涂覆的蚕丝和薇乔缝线记录了14天后释放的最低量的Ag离子，为0.03-0.45 ppm，而聚乙烯醇-银（PVA-Ag）涂覆的缝线显示出最高速率，为0.75-0.93 ppm。
Upgrading the surgical sutures, as the main wound closure device, is essential. The evolution of bacterial resistance and the plummeting of antibiotics have directed research toward augmented sutures. Nanotechnology has provided answers to these concerns. The use of bacterial isolates as bio-factory for synthesized silver nanoparticles (AgNPs) and silver nanocomposites via a one pot ex situ method provides environmentally friendly silver nanocomposites in addition to the use of chitosan and polyvinyl alcohol polymers as carriers. Transmission electron microscopy (TEM) and zeta potential analysis revealed spherical negatively charged AgNPs. These nanoparticles and nanocomposites were used as coatings for absorbable vicryl and nonabsorbable silk surgical sutures. Atomic force microscopy (AFM) 3D images of these coated sutures showed a significant decrease in surface roughness with improved surface topography, specifically with chitosan-silver (CS-Ag) vicryl coated sutures with effective attachment of the nanocomposite and nanoparticles thin film on the suture surface. Field emission scanning electron microscopy with energy dispersive x-ray spectroscopy (FE-SEM/EDX) analysis showed the significant presence of the thin film of coating materials on the surface of the sutures and the significant elemental presentation of Ag. Vicryl and silk coated CS-Ag sutures showed significant antibacterial and antibiofilm activities against both gram positive and gram-negative bacterial isolates. AgNPs coated silk and vicryl sutures recorded the lowest amounts of Ag ions at 0.03–0.45 ppm released after 14 days, while polyvinyl alcohol-silver (PVA-Ag) coated ones showed the highest rates at 0.75–0.93 ppm.