Metabolism and neurite promoting effect of exogenous sphingosylphosphocholine in cultured murine neuroblastoma cells.
Metabolism and neurite promoting effect of exogenous sphingosylphosphocholine in cultured murine neuroblastoma cells.
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DOI：
10.1093/oxfordjournals.jbchem.a124068
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发表时间：
1993-04
影响因子：
2.7
通讯作者：
E. Sugiyama;K. Uemura;A. Hara;T. Taketomi
中科院分区：
文献类型：
--
作者：
E. Sugiyama;K. Uemura;A. Hara;T. Taketomi
外源性鞘氨醇磷酸胆碱类似物和天然存在的鞘磷脂刺激神经突生长在培养的小鼠神经母细胞瘤细胞系，NS-20 Y，Neuro 2a，和N1 E-115，而外源性鞘氨醇在非细胞毒性浓度抑制神经突生长在NS-20 Y和Neuro 2a细胞。鞘氨醇磷酸胆碱对神经突生长的影响是可逆的，表明延长的神经突需要通过持续刺激来维持。在脉冲和追逐实验中的外源性[3- 3 H]鞘氨醇磷酸胆碱的摄取和代谢表明，放射性神经酰胺和鞘磷脂，这是检测到的主要代谢产物，是在前体/产品的关系。因此，假设细胞摄取的外源性鞘氨醇磷酸胆碱首先降解为磷酸胆碱和鞘氨醇，后者迅速酰化为神经酰胺，然后通过磷酸胆碱转移转化为鞘磷脂。神经鞘磷脂的合成可能与神经突起的生长有关。
Exogenous sphingosylphosphocholine analogues and naturally occurring sphingomyelin stimulated the neurite outgrowth in cultured murine neuroblastoma cell lines, NS-20Y, Neuro2a, and N1E-115, whereas exogenous sphinganine at a non-cytotoxic concentration inhibited the neurite outgrowth in NS-20Y and Neuro2a cells. The effect of sphingosylphosphocholine on the neurite outgrowth was reversible, indicating that the extended neurites needed to be maintained by continuous stimulation. The uptake and metabolism of the exogenous [3-3H]sphingosylphosphocholine in pulse and chase experiments suggested that the radioactive ceramide and sphingomyelin, which were detected as major metabolic products, were in a precursor/product relationship. It is thus assumed that the exogenous sphingosylphosphocholine taken up by the cells is first degraded into phosphocholine and sphingosine, of which the latter is rapidly acylated to ceramide then converted to sphingomyelin by phosphocholine transfer. Metabolism of sphingosylphosphocholine through sphingomyelin synthesis in the cells may be associated with neurite outgrowth.