Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection.
Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection.
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水稻 Xa21 引发的基因和途径对于对抗白叶枯病感染至关重要
DOI：
10.1038/srep12165
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发表时间：
2015-07-17
影响因子：
4.6
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Peng H;Chen Z;Fang Z;Zhou J;Xia Z;Gao L;Chen L;Li L;Li T;Zhai W;Zhang W
水稻白叶枯病是一种毁灭性的水稻病害。Xa21基因对BB具有广泛和持久的抗性。我们通过多世代回交，将Xa21导入水稻品种L，获得了近等基因的抗枯萎病水稻9311/Xa21。利用下一代测序技术，我们对两个品种在水稻黄单胞菌PV感染前和感染后四天内的转录本进行了分析。米饭。已鉴定的差异表达(DE)基因和信号通路揭示了Xa21的功能。令人惊讶的是，在感染之前，在316个信号通路中的135个上的1,889个基因是介于9311/Xa21和9311之间的DE。这些Xa21介导的基本途径主要包括与基本物质和能量代谢有关的途径，以及许多与细胞分裂素等植物激素有关的途径，这表明Xa21在入侵前触发了能量、植物激素和资源在基本细胞活动之间的重新分配。与直觉相反的是，感染后，两种植物之间的DE基因仅为感染前的三分之一；除少数胁迫相关途径外，感染后受影响的途径构成了Xa21介导的基本途径的一小部分。这些结果表明，Xa21启动了至关重要的基因和信号通路，增强了其对细菌感染的抵抗力。
Rice bacterial blight (BB) is a devastating rice disease. The Xa21 gene confers a broad and persistent resistance against BB. We introduced Xa21 into Oryza sativa L ssp indica (rice 9311), through multi-generation backcrossing and generated a nearly isogenic, blight-resistant 9311/Xa21 rice. Using next-generation sequencing, we profiled the transcriptomes of both varieties before and within four days after infection of bacterium Xanthomonas oryzae pv. oryzae. The identified differentially expressed (DE) genes and signaling pathways revealed insights into the functions of Xa21. Surprisingly, before infection 1,889 genes on 135 of the 316 signaling pathways were DE between the 9311/Xa21 and 9311 plants. These Xa21-mediated basal pathways included mainly those related to the basic material and energy metabolisms and many related to phytohormones such as cytokinin, suggesting that Xa21 triggered redistribution of energy, phytohormones and resources among essential cellular activities before invasion. Counter-intuitively, after infection, the DE genes between the two plants were only one third of that before the infection; other than a few stress-related pathways, the affected pathways after infection constituted a small subset of the Xa21-mediated basal pathways. These results suggested that Xa21 primed critically important genes and signaling pathways, enhancing its resistance against bacterial infection.