α-melanocyte-stimulating hormone stimulates prolactin secretion through melanocortin-3 receptors expressed in mammotropes in the mouse pituitary
α-melanocyte-stimulating hormone stimulates prolactin secretion through melanocortin-3 receptors expressed in mammotropes in the mouse pituitary
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DOI：
10.1159/000071965
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发表时间：
2003-08-01
影响因子：
4.1
通讯作者：
Takahashi, S
中科院分区：
文献类型：
--
作者：
Matsumura, R;Takagi, C;Takahashi, S
啮齿动物垂体的中间叶参与调节前叶分泌催乳素（PRL）。在之前的研究中，我们证实了a-黑色素细胞刺激激素（α - msh）对培养小鼠垂体细胞中PRL释放和黑素皮质素-3受体（MC3-Rs）表达的刺激作用。本研究的目的是通过测定小鼠垂体前叶中表达mc3 - r细胞的细胞类型，阐明a-MSH是否直接通过mc3 - r刺激PRL释放。Northern blot分析显示，成年雄性和雌性小鼠垂体前叶和神经中间叶中存在一个2.7 kb的MC3-R mRNA转录本。通过原位杂交分析MC3-R mRNA，然后免疫细胞化学检测PRL或GH，对小鼠垂体中MC3-R mRNA和PRL或GH进行双细胞定位。MC3-R mRNA在大多数乳腺细胞和部分体细胞中检测到。α - msh增加了乳腺组织中PRL的释放并刺激了DNA复制，这些作用被MC3-R和MC4-R拮抗剂SHU9119阻断。这些结果表明，a-MSH通过MC3-Rs刺激PRL的释放和乳腺细胞的增殖，提示来自中间叶的a-MSH可以调节小鼠垂体中乳腺细胞的功能。版权所有(C) 2003 S. Karger AG，巴塞尔。
The intermediate lobe of rodent pituitaries is involved in the regulation of prolactin (PRL) secretion from the anterior lobe. In a previous study, we demonstrated the stimulatory effect of a-melanocyte-stimulating hormone (alpha-MSH) on PRL release and the expression of melanocortin-3 receptors (MC3-Rs) in cultured mouse pituitary cells. The aim of the present study was to clarify whether a-MSH directly stimulates PRL release through the MC3-Rs by determining the cell type of MC3-R-expressing cells in the mouse pituitary anterior lobe. Northern blot analysis revealed a 2.7-kb transcript for MC3-R mRNA in the anterior and neurointermediate lobes of pituitary glands of adult male and female mice. Dual cellular localization of MC3-R mRNA and PRL or growth hormone (GH) in the mouse pituitary glands was performed by in situ hybridization analysis of MC3-R mRNA followed by immunocytochemical detection of PRL or GH. MC3-R mRNA was detected in most mammotropes and some somatotropes. alpha-MSH increased PRL release and stimulated DNA replication in mammotropes, and these effects were blocked by SHU9119, an antagonist of MC3-R and MC4-R. These results indicate that a-MSH stimulates PRL release and proliferation of mammotropes through MC3-Rs, and suggest that a-MSH from intermediate lobes can regulate mammotrope functions in the mouse pituitary. Copyright (C) 2003 S. Karger AG, Basel.