WGA-Alexa Conjugates for Axonal Tracing.
WGA-Alexa Conjugates for Axonal Tracing.
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DOI：
10.1002/cpns.28
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发表时间：
2017-04-10
影响因子：
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通讯作者：
Sillitoe, Roy V
中科院分区：
文献类型：
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作者：
Levy, Sabrina L;White, Joshua J;Sillitoe, Roy V
解剖标记方法对于理解大脑组织至关重要。这些方法中有多种执行纤维束追踪的方法。然而，在体内标记神经元时需要克服的一个主要障碍是可见性。能见度差使得对所需的神经元通路进行成像变得具有挑战性，从而可以轻松地将其与紧密相邻的通路区分开来。因此，分析单个预测或其联系变得不可能。为给定目的选择的示踪剂对示踪质量有重大影响。在这里，我们描述了与 Alexa 荧光团结合的麦芽凝集素 (WGA) 示踪剂，用于可靠地高分辨率追踪中枢神经系统预测。使用小鼠小脑作为模型系统，我们实施 WGA-Alexa 追踪来标记和映射控制运动功能的神经回路。我们还展示了其在体内进行单单元细胞外记录后标记小脑局部区域的实用性。 © 2017 John Wiley & Sons, Inc. 版权所有
Anatomical labeling approaches are essential for understanding brain organization. Among these approaches are various methods of performing tract tracing. However, a major hurdle to overcome when marking neurons in vivo is visibility. Poor visibility makes it challenging to image a desired neuronal pathway so that it can be easily differentiated from a closely neighboring pathway. As a result, it becomes impossible to analyze individual projections or their connections. The tracer that is chosen for a given purpose has a major influence on the quality of the tracing. Here, we describe the wheat germ agglutinin (WGA) tracer conjugated to Alexa fluorophores for reliable high-resolution tracing of central nervous system projections. Using the mouse cerebellum as a model system, we implement WGA-Alexa tracing for marking and mapping neural circuits that control motor function. We also show its utility for marking localized regions of the cerebellum after performing single-unit extracellular recordings in vivo. © 2017 by John Wiley & Sons, Inc.