Critical aggregation concentrations of gram-negative bacterial lipopolysaccharides (LPS)
Critical aggregation concentrations of gram-negative bacterial lipopolysaccharides (LPS)
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DOI：
10.1006/bbrc.1998.9773
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发表时间：
1998-12-09
影响因子：
3.1
通讯作者：
Wistrom, AO
中科院分区：
文献类型：
--
作者：
Aurell, CA;Wistrom, AO
细菌内毒素或脂多糖（LPS），革兰氏阴性菌的细胞壁成分，参与感染性休克。虽然过去已经研究了LPS的碳水化合物结构，但对血液中LPS片段的大分子结构和形成知之甚少。据信，两亲分子如LPS作为单体存在，并在临界胶束或临界聚集体浓度CAC以上聚集成大分子结构。通过静态光散射和稳态荧光光谱法，通过掺入荧光探针NPN（N-苯基-1-萘胺），建立了脂质A（LPS前体）和不同分子量和不同多糖链长度的几种LPS血清型的CAC。短链多糖突变体LPS S的CAC。miniRes Re 595（MW 2，500）和来自S.最小分子量Re 595（MW 2000）分别为4 μ M和5 μ M。对异质性长O抗原多糖链细菌血清型S.野生型的最低浓度为11 μ g LPS/ml，S.鼠伤寒沙门氏菌14 μ g LPS/ml和大肠杆菌0111：B4 22 μ g LPS/ml。结果表明，LPS的临界聚集浓度和溶解度是多糖链长的函数。(C)北京：科学出版社.
Bacterial endotoxins or lipopolysaccharides (LPS), cell wall components of gram negative bacteria, are involved in septic shock. While the carbohydrate structure of LPS have been studied in the past, little is known about the macromolecular structure and formation of LPS fragments in blood. It is believed that amphiphilic molecules such as LPS occur as monomers and aggregate into macromolecular structures above a critical micelle or critical aggregate concentration, CAC. The CAC of Lipid A, a LPS precursor, and several LPS serotypes of varying molecular weight and different polysaccharide chain lengths were established by static light scattering and by steady-state fluorescence spectroscopy by incorporation of the fluorescent probe, NPN, N-phenyl-1-naphthylamine. The CAC for short polysaccharide chain mutant LPS S. minnesota Re 595 (MW 2,500) and Lipid A from S. minnesota Re 595 (MW 2000) were 4 mu M, and 5 mu M respectively. The CAC of LPS from heterogenous long O-antigen polysaccharide chain bacterial serotypes: S. minnesota wildtype were 11 mu g LPS/ml, S. typhimurium 14 mu g LPS/ml and E.coli 0111:B4 22 mu g LPS/ml, respectively. The result obtained suggests that critical aggregate concentration and solubility of LPS is a function of polysaccharide chain length. (C) 1998 Academic Press.