Identification of Significant Pathways Induced by PAX5 Haploinsufficiency Based on Protein-Protein Interaction Networks and Cluster Analysis in Raji Cell Line.
Identification of Significant Pathways Induced by PAX5 Haploinsufficiency Based on Protein-Protein Interaction Networks and Cluster Analysis in Raji Cell Line.
复制标题
基于 Raji 细胞系中蛋白质-蛋白质相互作用网络和聚类分析鉴定 PAX5 单倍体不足诱导的重要途径
DOI：
10.1155/2017/5326370
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发表时间：
2017
影响因子：
--
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Gu J;Li T;Zhao L;Liang X;Fu X;Wang J;Shang Z;Huang W;Zhou J
PAX5编码一种对b细胞分化至关重要的转录因子，PAX5单倍性不足与肿瘤发生有关。关于PAX5单倍不全如何调控基因表达促进肿瘤发生的研究很少。本研究利用基因编辑技术在Raji细胞中构建PAX5单倍不全的细胞模型，检测PAX5单倍不全Raji细胞中差异表达的基因，并利用蛋白-蛋白相互作用网络和聚类分析全面探讨PAX5单倍不全的细胞通路。在Raji细胞中，基因转录簇、炎症和免疫反应簇以及癌症通路被确定为与PAX5单倍体功能不全相关的三个重要通路。这些变化提示PAX5单倍不足促进肿瘤发生的机制可能与基因组不稳定性、免疫耐受和肿瘤通路有关。
PAX5 encodes a transcription factor essential for B-cell differentiation, and PAX5 haploinsufficiency is involved in tumorigenesis. There were few studies on how PAX5 haploinsufficiency regulated genes expression to promote tumorigenesis. In this study, we constructed the cell model of PAX5 haploinsufficiency using gene editing technology in Raji cells, detected differentially expressed genes in PAX5 haploinsufficiency Raji cells, and used protein-protein interaction networks and cluster analysis to comprehensively investigate the cellular pathways involved in PAX5 haploinsufficiency. The clusters of gene transcription, inflammatory and immune response, and cancer pathways were identified as three important pathways associated with PAX5 haploinsufficiency in Raji cells. These changes hinted that the mechanism of PAX5 haploinsufficiency promoting tumorigenesis may be related to genomic instability, immune tolerance, and tumor pathways.