An Inexpensive and Effective Larval Diet for Anopheles arabiensis (Diptera: Culicidae): Eat Like a Horse, a Bird, or a Fish?
An Inexpensive and Effective Larval Diet for Anopheles arabiensis (Diptera: Culicidae): Eat Like a Horse, a Bird, or a Fish?
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DOI：
10.1603/me11289
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发表时间：
2012-09-01
影响因子：
2.1
通讯作者：
Gilles, J. R. L.
中科院分区：
文献类型：
--
作者：
Damiens, D.;Benedict, M. Q.;Gilles, J. R. L.
成功的昆虫不育技术计划取决于大规模生产高质量的不育昆虫，并将其释放到目标地区。在苏丹北方的一个试验区，为控制阿拉伯按蚊，每天需要阿拉伯不育雄性。为了达到这一生产目标，蚊子的大量饲养是必不可少的，幼虫的饮食，生产健康的雄性蚊子的关键参数，需要具有成本效益。锦鲤漂浮混合鱼食，以前在粮食及农业组织/国际原子能机构昆虫害虫控制实验室用于常规饲养的An。Arabiensis colony，不再可用。这项研究的目的是找到一种便宜而有效的替代品。测试了几种市售的候选粉末状饮食以确定最佳饮食。采用混料试验原理和响应面分析法，以牛肝粉和金枪鱼粉两种组分的组合，在幼虫存活率、发育率和成虫大小方面均表现出最佳效果。添加维生素混合物进一步改善了饮食。这些积极的生产结果加上我们的混合物相对较低的成本证明了其用于大规模饲养目的的可能性。
A successful sterile insect technique program depends upon mass production of good-quality sterile insects for release into a target area. Specifically, to control Anopheles arabiensis Patton (Diptera: Culicidae) in a pilot area in northern Sudan, 1 million An. arabiensis sterile males per day are needed. To reach this production objective, mosquito mass rearing is indispensable and larval diet, a key parameter for the production of healthy male mosquitoes, needs to be cost-effective. The Koi Floating Blend fish food, previously used at the Food and Agriculture Organization/International Atomic Energy Agency Insect Pest Control Laboratory for the routine rearing of the An. arabiensis colony, is no longer available. The aim of this study was to find a cheap and effective substitute for the discontinued diet. Several candidate powdered diets that are commercially available were tested to determine the best diet. By using mixture experiment principles and response surface methodology, the combination of two components, bovine liver powder and tuna meal, showed the best results in terms of larval survival, developmental, rate and adult size. The addition of a vitamin mixture further improved the diet. These positive production results coupled with the relatively low cost of our blend demonstrated the possibility of its use for mass rearing purpose.