So many roads traveled: A career in science and administration.
So many roads traveled: A career in science and administration.
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走过了那么多路：科学和管理领域的职业生涯。
DOI：
10.1074/jbc.x119.012206
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Halpert,JamesR
中科院分区：
文献类型：
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作者：
Halpert,JamesR
在我的职业生涯中，我走过了很多条路。在洛杉矶度过了我的头19年后，我成了某种程度上的学术流浪者，在美国的六所大学和瑞典的三所大学学习和/或工作。按照时间顺序，我在加州大学洛杉矶分校获得了斯堪的纳维亚语言和文学学士学位，在乌普萨拉大学获得了生物化学博士学位，在卡罗林斯卡研究所获得了毒理学硕士学位。我曾在自然科学、药学和医学学院工作过，并在多个基础科学系和一个临床科工作过。我曾担任过研究跟踪和终身教员、系主任、副院长和院长。我的研究涵盖了毒物学、生物化学、毒理学和药理学。通过所有的动作，我得到了很多，也失去了一些。在过去的40年里，我一直对细胞色素P450的结构-功能和结构-活性关系感兴趣。我的实验室一直专注于使用X射线结晶学、定点突变、氢交换MS、等温滴定量热法和结合各种功能分析的计算方法来研究CYP2B酶。这一全面的方法使人们能够详细了解CYP2B酶显着底物混杂的结构基础。我们还利用生物物理和先进的光谱技术研究了CYP3A4变构的机制，发现了P450-P450相互作用和多配体结合的关键作用。这篇文章的一个主要目标是为处于职业生涯早期和中期的科学家以及那些正在考虑管理变动的高级科学家提供可能有用的经验教训。
I have traveled many roads during my career. After spending my first 19 years in Los Angeles, I became somewhat of an academic nomad, studying and/or working in six universities in the United States and three in Sweden. In chronological order, I have a B.A. in Scandinavian languages and literature from UCLA, a Ph.D. in biochemistry from Uppsala University, and an M.S. in toxicology from the Karolinska Institute. I have been in schools of natural science, pharmacy, and medicine and have worked in multiple basic science departments and one clinical department. I have served as a research-track and tenured faculty member, department chair, associate dean, and dean. My research has spanned toxinology, biochemistry, toxicology, and pharmacology. Through all the moves, I have gained much and lost some. For the past 40 years, my interest has been cytochrome P450 structure-function and structure-activity relationships. My lab has focused on CYP2B enzymes using X-ray crystallography, site-directed mutagenesis, deuterium-exchange MS, isothermal titration calorimetry, and computational methods in conjunction with a variety of functional assays. This comprehensive approach has enabled detailed understanding of the structural basis of the remarkable substrate promiscuity of CYP2B enzymes. We also have investigated the mechanisms of CYP3A4 allostery using biophysical and advanced spectroscopic techniques, and discovered a pivotal role of P450-P450 interactions and of multiple-ligand binding. A major goal of this article is to provide lessons that may be useful to scientists in the early and middle stages of their careers and those more senior scientists contemplating an administrative move.