Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet
Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet
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DOI：
10.1159/000335807
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发表时间：
2011-01-01
影响因子：
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通讯作者：
Yun, Jong Won
中科院分区：
文献类型：
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作者：
Choi, Duk Kwon;Oh, Tae Seok;Yun, Jong Won
背景资料：虽然性别作为健康和疾病的关键决定因素的重要性早已得到承认，但仍然缺乏对医学中性别差异的系统研究。我们假设，肩胛间棕色脂肪细胞组织（BAT），不仅是一个关键的组织，有助于能量消耗，但也调节饮食诱导的产热，并可能是一个理想的目标，研究性别差异的肥胖发展响应高脂肪饮食（HFD）。研究方法：因此，我们进行了差异蛋白质组分析的BAT从瘦和肥胖的两种性别的大鼠喂食HFD使用2-DE结合MALDI-TOF-MS。结果：当暴露于HFD，雄性大鼠体重增加，血浆生化参数的值比雌性大鼠增加。在595个匹配的点中，48个差异表达的鉴定点显示出显著的性别差异，而7个蛋白质没有显示出性别差异，但确实显示出HFD反应。结论：蛋白质组学研究性别二态蛋白调制BAT可能会提供结论性的结果，显示较高的表达的许多蛋白质参与产热和脂肪氧化，以及较低的表达的蛋白质有助于脂肪合成的雌性大鼠比雄性大鼠。版权所有（C）2011 S. Karger AG，巴塞尔
Background: Although the importance of gender as a key determinant in health and illness has been recognized for a long time, systematic studies of gender differences in medicine are still lacking. We hypothesized that interscapular brown adipocyte tissue (BAT), is not only a key tissue contributing to energy expenditure, but also regulates diet-induced thermogenesis, and may be an ideal target for studying gender differences in obesity development in response to a high fat diet (HFD). Methods: We therefore performed differential proteome analysis of BAT from lean and obese rats of both genders fed a HFD using 2-DE combined with MALDI-TOF-MS. Results: When exposed to a HFD, male rats gained more body weight with increased values of plasma biochemical parameters than did female rats. Among 595 matched spots, 48 differentially expressed identified spots showed significant gender differences, whereas 7 proteins showed no gender differences, but did show a HFD response. Conclusions: Proteomic investigations into gender-dimorphic protein modulation in BAT may provide conclusive results showing higher expression of numerous proteins involved in thermogenesis and fat oxidation as well as lower expression of proteins contributing to fat synthesis in female rats than in male rats. Copyright (C) 2011 S. Karger AG, Basel