Chemical Preconditioning with 3-Nitropropionic Acid in Gerbil Hippocampal Slices: Therapeutic Window and the Participation of Adenosine Receptor
Chemical Preconditioning with 3-Nitropropionic Acid in Gerbil Hippocampal Slices: Therapeutic Window and the Participation of Adenosine Receptor
复制标题
沙鼠海马切片中 3-硝基丙酸的化学预处理：治疗窗口和腺苷受体的参与
作者：
S. Aketa;H. Nakase;Y. Kamada;Ken;T. Sakaki
亚毒性剂量的神经毒素3-硝基丙酸（3-NPA）诱导的缺血耐受最近被报道为“化学预处理”。"我们使用耐受现象的沙鼠海马切片模型，从电生理学角度研究了腺苷受体的治疗窗口和作用效果。3-在切片制备前3、24和72 h腹腔内给予4 mg/kg剂量的NPA。与对照组相比，缺氧3h和24h预处理组的缺氧去极化（HD）延迟时间延长（P <0.05），缺氧8min后的兴奋性突触后电位恢复明显改善（P <0.05）。HD延迟时间与恢复时间呈高度相关（r = 0.37，P <0.001）。这些作用完全逆转茶碱（20毫克/公斤），腺苷受体阻滞剂的管理。这些结果表明，3-NPA化学预处理诱导的早期发作（3小时）和持久（24小时）的耐受缺氧损伤兴奋性突触机制在海马通过延迟钙内流，腺苷受体的激活有助于这种神经保护作用。
Ischemic tolerance induced by a subtoxic dose of neurotoxin, 3-nitropropionic acid (3-NPA), was recently reported as "chemical preconditioning." We electrophysiologically investigated the therapeutic window and the effect via action at the adenosine receptor using a gerbil hippocampal slice model of the tolerance phenomenon. 3-NPA at the dose of 4 mg/kg was administered intraperitoneally at 3, 24, and 72 h prior to slice preparation. Prolonged delay to hypoxic depolarization (HD) and improvement of the field excitatory postsynaptic potential recovery following a fixed period of hypoxia (8 min) were observed in the groups pretreated at 3 and 24 h compared with the control (P < 0.05). Correlation between the delay to HD and the recovery was highly significant (r = 0.37, P < 0.001). These effects were completely reversed by administration of theophylline (20 mg/kg), an adenosine receptor blocker. These findings indicate that chemical preconditioning with 3-NPA induces early onset (3 h) and long-lasting (24 h) tolerance of hypoxic damage to excitatory synaptic mechanisms in the hippocampus by delayed calcium entry, and the activation of adenosine receptor contributes to this neuroprotective effect.
神经元的抑郁样去极化和选择性脆弱性的传播。
影响因子：
8.3
作者：
Somjen,GG;Aitken,PG;Balestrino,M;Herreras,O;Kawasaki,K
通讯作者：
Kawasaki,K