Genome-wide analysis of mRNAs targeted to yeast mitochondria
Genome-wide analysis of mRNAs targeted to yeast mitochondria
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DOI：
10.1093/embo-reports/kvf025
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发表时间：
2002-02-01
期刊：
影响因子：
7.7
通讯作者：
Jacq, C
中科院分区：
文献类型：
--
作者：
Marc, P;Margeot, A;Jacq, C
人们一致认为，核编码的线粒体蛋白是翻译后靶向线粒体的，即使在某些情况下，共翻译阶段可以帮助前体蛋白的导入。我们使用酵母 DNA 微阵列来分析与游离和线粒体结合的多核糖体相关的 mRNA 群体。正如预期的那样，许多已知编码线粒体蛋白的 mRNA 定位于游离的细胞质多核糖体，但许多 mRNA 定位于线粒体结合的多核糖体。此外，六个随机选择的线粒体结合 mRNA 的 3'-UTR 包含足够的信息，可以在体内将不可翻译的 RNA 靶向线粒体附近。有趣的是，产生靶向线粒体的 mRNA 的基因主要源自古代细菌，而产生在细胞质中翻译的 mRNA 的基因则主要源自真核生物。这些观察结果支持了最近有关线粒体蛋白质组双重起源的假设，为线粒体的生物发生提供了新的见解。
It is agreed that nuclear-encoded mitochondrial proteins are post-translationally targeted to mitochondria, even if, in some cases, a co-translational phase can assist the import of precursor proteins. We used yeast DNA microarrays to analyse the mRNA populations associated with free and mitochondrion-bound polysomes. As expected, many mRNAs, known to encode mitochondrial proteins, are localized to free cytoplasmic polysomes, but many are localized to mitochondrion-bound polysomes. Furthermore, the 3'-UTR of six randomly chosen mitochondrion-bound mRNAs contains sufficient information to target, in vivo, non-translatable RNA to the vicinity of mitochondria. Interestingly, genes producing mRNAs that are targeted to mitochondria are mainly of ancient bacterial origin, whereas those producing mRNAs that are translated in the cytoplasm are mainly of eukaryotic origin. These observations, which support the recent hypotheses concerning the dual origin of the mitochondrial proteome, provide new insights into the biogenesis of mitochondria.