Disposable polydimethylsiloxane/silicon hybrid chips for protein detection
Disposable polydimethylsiloxane/silicon hybrid chips for protein detection
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DOI：
10.1016/j.bios.2004.12.010
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发表时间：
2005-10-15
影响因子：
12.6
通讯作者：
Chen, SC
中科院分区：
文献类型：
--
作者：
Li, SF;Floriano, PN;Chen, SC
本文介绍了一种由聚二甲基硅氧烷(PDMS)和硅构成的单室或四室一次性蛋白质分析芯片。这些芯片由夹在硅微芯片中的PDMS层的多层堆叠组成。这种内部硅芯片的特点是蚀刻了一系列容纳聚合物珠子的微腔。样品通过最上面的PDMS层被引入流体网络，在那里它被引导到珠室。分析物与探针珠反应后，在探针珠上产生的信号被CCD摄像机捕捉、数字处理和分析。建立了一种基于微珠的C-反应蛋白(CRP)荧光分析方法，用于表征这些混合芯片。单室蛋白质芯片的检测下限为1 ng/ml。此外，采用背压补偿法，四室芯片各室信号之间的误差在10%以内。(C)2004爱思唯尔B.V.保留所有权利。
This paper presents disposable protein analysis chips with single- or four-chamber-constructed from poly(dimethylsiloxane) (PDMS) and silicon. The chips are composed of a multilayer stack of PDMS layers that sandwich a silicon microchip. This inner silicon chip features an etched array of microcavities hosting polymeric beads. The sample is introduced into the fluid network through the top PDMS layer, where it is directed to the bead chamber. After reaction of the analyte with the probe beads, the signal generated on the beads is captured with a CCD camera, digitally processed, and analyzed. An established bead-based fluorescent assay for C-reactive protein (CRP) was used here to characterize these hybrid chips. The detection limit of the single-chamber protein chip was found to be 1 ng/ml. Additionally, using a back pressure compensation method, the signals from each chamber of the four-chamber chip were found to fall within 10% of each other. (c) 2004 Elsevier B.V. All rights reserved.