Binding sites in mammalian genes and viral gene regulatory regions recognized by methylated DNA-binding protein.
Binding sites in mammalian genes and viral gene regulatory regions recognized by methylated DNA-binding protein.
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DOI：
10.1093/nar/18.21.6253
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发表时间：
1990-11
影响因子：
14.9
通讯作者：
X. Y. Zhang;C. Asiedu;P. C. Supakar;R. Khan;K. Ehrlich;M. Ehrlich
中科院分区：
文献类型：
--
作者：
X. Y. Zhang;C. Asiedu;P. C. Supakar;R. Khan;K. Ehrlich;M. Ehrlich
甲基化DNA结合蛋白（MDBP）是一种普遍存在的哺乳动物蛋白，它识别多种相关的DNA序列。这些序列中的一些需要其CpG二核苷酸的甲基化以用于结合，而其他序列不需要。我们报告说，MDBP结合，在DNA甲基化独立的方式，在小鼠多瘤病毒增强子的两个网站，一个在增强的人肝炎B病毒，并在马传染性贫血前病毒DNA的长末端重复。我们还发现，在人类和啮齿类动物DNA中的许多MDBP位点，当它们在识别位点内的CpG二核苷酸处被甲基化时，它们与MDBP的结合要好得多。这些位点包括次黄嘌呤磷酸核糖转移酶、HLA-A2、-A3和-A25抗原以及α-半乳糖苷酶A的人类基因起始位点。在甲基化反应MDBP位点的情况下，在分化或DNA复制过程中其甲基化状态的变化可以通过调节转录来帮助驱动发育。
Methylated DNA-binding protein (MDBP), a ubiquitous mammalian protein, recognizes a variety of related DNA sequences. Some of these sequences require methylation of their CpG dinucleotides for binding and others do not. We report that MDBP binds, in a DNA methylation-independent fashion, to two sites in the mouse polyomavirus enhancer, one in the enhancer of the human hepatitis B virus, and to one in the long terminal repeat of equine infectious anemia proviral DNA. We have also found a number of MDBP sites in human and rodent DNAs which bind much better to MDBP when they are methylated at CpG dinucleotides within the recognition site. These include sites at the beginning of the human genes for hypoxanthine phosphoribosyl transferase, HLA-A2, -A3, and -A25 antigens, and alpha-galactosidase A. In the case of methylation-responsive MDBP sites, changes in their methylation status during differentiation or DNA replication could help drive development by modulating transcription.