Photorefractive keratectomy in the cat eye: biological and optical outcomes.
Photorefractive keratectomy in the cat eye: biological and optical outcomes.
复制标题
猫眼屈光性角膜切除术：生物学和光学结果。
DOI：
10.1016/j.jcrs.2007.02.021
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发表时间：
2007
影响因子：
2.8
通讯作者：
Huxlin,KrystelR
中科院分区：
文献类型：
--
作者：
Nagy,LanaJ;MacRae,Scott;Yoon,Geunyoung;Wyble,Matthew;Wang,Jianhua;Cox,Ian;Huxlin,KrystelR
目的量化光屈光性角膜切除术（PRK）前后猫角膜的光学和生物力学特性，评估不同生物因素对屈光性结果的相对贡献。美国纽约州罗切斯特市罗彻斯特大学眼科。方法成年猫6.0屈光度(D)近视眼或4.0 D远视眼PRK超过6.0或8.0 mm光学区（OZ）。测量术前和术后波前像差、眼压（IOP）、角膜迟滞、角膜阻力因子、眼轴长度、角膜厚度和曲率半径。最后，对尸后波形蛋白和α-平滑肌肌动蛋白进行免疫组化。结果光屈光性角膜切除术改变了猫的眼部散焦，增加了高阶像差，并诱导了肌成纤维细胞分化。然而，预期的散焦校正仅在8.0 mm OZs下实现。在近视眼后发现上皮和间质表面长期变平，而在远视后则没有。眼压未受PRK影响；然而，角膜迟滞和角膜阻力因子降低。在随后的6个月里，眼像差和IOP保持稳定，而角膜中央厚度、角膜迟滞和角膜阻力因子趋于正常水平。结论：scat角膜对PRK表现出光学、组织学和生物力学反应，与先前在人类中描述的相似，特别是当OZ大小归一化到角膜总面积时。然而，猫表现出明显的基质再生，导致术前角膜厚度、角膜迟滞和角膜阻力因子的恢复，而手术引起的光学变化没有明显的消退。因此，尽管角膜生物学存在明显的物种差异，激光屈光手术对眼波前像差的主要影响是可以实现的。
PURPOSETo quantify optical and biomechanical properties of the feline cornea before and after photorefractive keratectomy (PRK) and assess the relative contribution of different biological factors to refractive outcome.SETTINGDepartment of Ophthalmology, University of Rochester, Rochester, New York, USA.METHODSAdult cats had 6.0 diopter (D) myopic or 4.0 D hyperopic PRK over 6.0 or 8.0 mm optical zones (OZ). Preoperative and postoperative wavefront aberrations were measured, as were intraocular pressure (IOP), corneal hysteresis, the corneal resistance factor, axial length, corneal thickness, and radii of curvature. Finally, postmortem immunohistochemistry for vimentin and α-smooth muscle actin was performed.RESULTSPhotorefractive keratectomy changed ocular defocus, increased higher-order aberrations, and induced myofibroblast differentiation in cats. However, the intended defocus corrections were only achieved with 8.0 mm OZs. Long-term flattening of the epithelial and stromal surfaces was noted after myopic, but not after hyperopic, PRK. The IOP was unaltered by PRK; however, corneal hysteresis and the corneal resistance factor decreased. Over the ensuing 6 months, ocular aberrations and the IOP remained stable, while central corneal thickness, corneal hysteresis, and the corneal resistance factor increased toward normal levels.CONCLUSIONSCat corneas exhibited optical, histological, and biomechanical reactions to PRK that resembled those previously described in humans, especially when the OZ size was normalized to the total corneal area. However, cats exhibited significant stromal regeneration, causing a return to preoperative corneal thickness, corneal hysteresis and the corneal resistance factor without significant regression of optical changes induced by the surgery. Thus, the principal effects of laser refractive surgery on ocular wavefront aberrations can be achieved despite clear interspecies differences in corneal biology.