DEVELOPMENTAL EXPRESSION OF GLUTATHIONE-S-TRANSFERASE IN MAIZE AND ITS POSSIBLE CONNECTION WITH HERBICIDE TOLERANCE
DEVELOPMENTAL EXPRESSION OF GLUTATHIONE-S-TRANSFERASE IN MAIZE AND ITS POSSIBLE CONNECTION WITH HERBICIDE TOLERANCE
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DOI:
10.1007/bf00040624
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发表时间:
1993-01-01
期刊:
影响因子:
1.9
通讯作者:
VILLA, M
中科院分区:
文献类型:
--
作者:
SARIGORLA, M;FERRARIO, S;VILLA, M
Crop improvement for tolerance to specific herbicides is an important breeding target, since molecules performing well with regard to environmental safety are frequently not completely selective for crops. The glutathione (GSH)/glutathione-S-transferase (GST) system is a general mechanism of detoxification that in higher plants may confer tolerance to some herbicides. GSH level and GST activity were measured in different maize inbred lines, in the absence or in the presence of EPTC (a thiocarbamate) and of Alachlor (a chloroacetanilide); a wide genetic variability was observed for these parameters, which appear to be involved in plant tolerance to herbicides. Isozyme analysis was performed on roots, leaves, scutellum, pollen, coleoptile, mesocotyl of the same inbreds: it revealed the presence of many GST forms in maize, showing high polymorphism; they are controlled by at least five genes, the expression of which is developmentally regulated in the different tissues analyzed.