Noise magnetic fields abolish the gap junction intercellular communication suppression induced by 50 hz magnetic fields
Noise magnetic fields abolish the gap junction intercellular communication suppression induced by 50 hz magnetic fields
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DOI：
10.1002/bem.20207
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发表时间：
2006-05
影响因子：
1.9
通讯作者：
Q. Zeng;X. Ke;Xiangwei Gao;Yi-ti Fu;D. Lu;H. Chiang;Zheng-ping Xu
中科院分区：
文献类型：
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作者：
Q. Zeng;X. Ke;Xiangwei Gao;Yi-ti Fu;D. Lu;H. Chiang;Zheng-ping Xu
在此之前，我们已经报道，暴露于50赫兹相干正弦磁场(MF)24小时，在0.4mT强度下可抑制哺乳动物细胞间隙连接通讯(GJIC)，而在0.2mT强度下可增强12-O-十四烷基佛波醇-13-乙酸酯(TPA)的抑制作用。在本研究中，我们进一步探讨了非相干噪声磁场对磁场诱导的GJIC抑制的影响。用激光扫描共聚焦显微镜检测GJIC。以光漂白后10min的荧光恢复率(R)作为GJIC的功能指标。经0.4mT、50 Hz正弦磁场作用24 h后，NIH3T3细胞的R值为30.85±14.70%，而假暴露组的R值为46.36±20.68%，表明磁场对NIH3T3细胞的GJIC有明显的抑制作用(P<0.05)，说明叠加噪声的Mf可减轻50 HzMF对GJIC的抑制作用。此外，尽管0.2mT的噪声磁场能协同增强TPA诱导的GJIC抑制作用(R：24.90±13.50%比35.82±17.18%，P<；0.05)，但进一步施加噪声磁场可消除相干磁场的协同作用(R：32.51±18.37%)。总体而言，目前的数据清楚地表明，尽管噪声磁场本身对NIH3T3细胞的GJIC没有影响，但它叠加在相同强度的相干正弦磁场上，取消了MF诱导的GJIC抑制。这是第一个使用信号成分作为测试终点的噪声中和中和50赫兹中频生物效应的报告。《生物电磁学》27：274-279,2006。©2006 Wiley-Liss，Inc.
Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) at 0.2 mT. In the present study, we further explored the effects of incoherent noise MF on MF‐induced GJIC inhibition. GJIC was determined by fluorescence recovery after photobleaching (FRAP) with a laser‐scanning confocal microscope. The rate of fluorescence recovery (R) at 10 min after photobleaching was adopted as the functional index of GJIC. The R‐value of NIH3T3 cells exposed to 50 Hz sinusoidal MF at 0.4 mT for 24 h was 30.85 ± 14.70%, while the cells in sham exposure group had an R‐value of 46.36 ± 20.68%, demonstrating that the GJIC of NIH3T3 cells was significantly inhibited by MF exposure (P .05), indicating that the superposition of a noise MF alleviated the suppression of GJIC induced by the 50 Hz MF. In addition, although MF at an intensity of 0.2 mT synergistically enhanced TPA‐induced GJIC inhibition (R: 24.90 ± 13.50% vs. 35.82 ± 17.18%, P < .05), further imposition of a noise MF abolished the synergistic effect of coherent MF (R: 32.51 ± 18.37%). Overall, the present data clearly showed that although noise MF itself had no effect on GJIC of NIH3T3 cells, its superposition onto a coherent sinusoidal MF at the same intensity abolished MF‐induced GJIC suppression. This is the first report showing that noise MF neutralizes 50 Hz MF‐induced biological effect by using a signaling component as the test endpoint. Bioelectromagnetics 27:274–279, 2006. © 2006 Wiley‐Liss, Inc.