Characterization of terfestatin A, a new specific inhibitor for auxin signaling
Characterization of terfestatin A, a new specific inhibitor for auxin signaling
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DOI：
10.1104/pp.105.068924
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发表时间：
2005-10-01
期刊：
影响因子：
7.4
通讯作者：
Nozaki, H
中科院分区：
文献类型：
--
作者：
Yamazoe, A;Hayashi, K;Nozaki, H
Terfestatin A（TrfA），三联苯-β-葡糖苷，是从链霉菌F40中分离的，用于正向筛选抑制拟南芥（Arabidopsis thaliana）中生长素诱导基因表达的化合物。TrfA特异性竞争性抑制拟南芥根中主要生长素诱导基因的表达，但不影响其他植物激素如脱落酸和细胞分裂素调控基因的表达。TrfA还阻断了生长素增强的生长素/吲哚-3-乙酸（Aux/IAA）阻遏蛋白的降解，而不影响生长素刺激的Aux/IAAs和F-box蛋白TIR 1之间的相互作用。TrfA处理拮抗生长素在根中的反应，包括主根抑制，侧根发生，根毛促进，和根向地性，但只有有限的影响，地上部生长素的反应。综上所述，这些结果表明，TrfA作为一个调节器的Aux/IAA的稳定性，从而提供了一个新的工具，解剖生长素信号。
Terfestatin A (TrfA), terphenyl-beta-glucoside, was isolated from Streptomyces sp. F40 in a forward screen for compounds that inhibit the expression of auxin-inducible genes in Arabidopsis (Arabidopsis thaliana). TrfA specifically and competitively inhibited the expression of primary auxin-inducible genes in Arabidopsis roots, but did not affect the expression of genes regulated by other plant hormones such as abscisic acid and cytokinin. TrfA also blocked the auxin-enhanced degradation of auxin/indole-3-acetic acid (Aux/IAA) repressor proteins without affecting the auxin-stimulated interaction between Aux/IAAs and the F-box protein TIR1. TrfA treatment antagonized auxin responses in roots, including primary root inhibition, lateral root initiation, root hair promotion, and root gravitropism, but had only limited effects on shoot auxin responses. Taken together, these results indicate that TrfA acts as a modulator of Aux/IAA stability and thus provides a new tool for dissecting auxin signaling.