Stimulation of central and systemic oxytocin release by histamine in the paraventricular hypothalamic nucleus: Evidence for an interaction with norepinephrine
Stimulation of central and systemic oxytocin release by histamine in the paraventricular hypothalamic nucleus: Evidence for an interaction with norepinephrine
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DOI：
10.1210/en.140.3.1158
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发表时间：
1999-03-01
期刊：
影响因子：
4.8
通讯作者：
Crowley, WR
中科院分区：
文献类型：
--
作者：
Bealer, SL;Crowley, WR
中枢组胺能神经元参与了包括分娩和哺乳在内的各种生理条件下催产素（OT）分泌的控制。本研究调查组胺是否也影响OT的中枢核内释放，这是已知的OT神经元的激活是重要的，以及组胺与去甲肾上腺素在全身和中枢OT释放的可能相互作用。将微透析探针紧邻下丘脑室旁核（PVN）放置，并用于给予人工脑脊液（ACSF）载体、含有组胺的ACSF、含有组胺与特异性H1或H2组胺受体拮抗剂组合的ACSF或含有组胺和α-肾上腺素能拮抗剂酚妥拉明的ACSF。收集透析液和血浆，并使用RIA测定OT浓度。用含有组胺的ACSF透析PVN显著增加了PVN内全身和中枢OT的释放。此外，透析液和血浆中OT浓度的增加被同时给予扑尔敏（一种H1受体拮抗剂）或雷尼替丁（一种H2受体拮抗剂）以及肾上腺素能拮抗剂酚妥拉明阻止。这些数据表明组胺在PVN内起作用以增加OT的全身和核内释放。此外，组胺诱导的OT释放增加依赖于H1和H2组胺能受体的刺激和随后的α-去甲肾上腺素能受体的激活。这些结果表明，组胺诱导全身和核内OT释放刺激释放去甲肾上腺素。
Central histaminergic neurons have been implicated in the control of oxytocin (OT) secretion in various physiological conditions, including parturition and lactation. The present studies investigated whether histamine also influences the central intranuclear release of OT, which is known to be important in the activation of OT neurons, and the possible interaction of histamine with norepinephrine in systemic and central OT release. Microdialysis probes were placed immediately adjacent to the hypothalamic paraventricular nucleus (PVN) and used for administration of artificial cerebrospinal fluid (ACSF) vehicle, ACSF containing histamine, ACSF containing histamine in combination with a specific H1 or H2 histamine receptor antagonist, or ACSF containing histamine and the alpha-adrenergic antagonist phentolamine. Dialysates and plasma were collected, and OT concentrations were determined using RIA. Dialysis of the PVN with ACSF containing histamine significantly increased the release of OT systemically and centrally within the PVN. Furthermore, the in creases in OT concentration in dialysates and plasma were prevented by simultaneous administration of chlorpheniramine (an H1 receptor antagonist) or ranitidine (an H2 receptor antagonist) as well as by the adrenergic antagonist phentolamine. These data demonstrate that histamine acts within the PVN to increase both systemic and intranuclear release of OT. Furthermore, the increased OT release induced by histamine is dependent upon stimulation of both H1 and H2 histaminergic receptors and subsequent activation of alpha-noradrenergic receptors. These findings suggest that histamine induces systemic and intranuclear OT release by stimulating the release of norepinephrine.