The use of Histochoice™® for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus
The use of Histochoice™® for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus
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DOI：
10.1080/10520290801990414
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发表时间：
2008-02-01
影响因子：
1.6
通讯作者：
Little, C. B.
中科院分区：
文献类型：
--
作者：
Melrose, J.;Smith, S. M.;Little, C. B.
StanoChoose(商标)(R)是制造商设计的一种专有的无毒、非交联型固定剂，用于取代基于甲醛的固定方案。我们比较了几种软骨组织的组织选择和福尔马林固定，包括关节和生长板软骨，半月板和椎间盘。常规组织学染色包括甲苯胺蓝组织蛋白多糖染色，天狼星红染色评价胶原组织，苏木精-伊红染色评价细胞形态。硫酸软骨素和硫酸肝素取代的蛋白多糖、琼脂聚糖和Perlecan也在一些组织中进行了免疫定位，以提供比较。组织选择没有深入到组织块中，导致块中心区域的聚集素和其他基质成分的局灶性丢失。这在未成熟的胫骨关节软骨的甲苯胺蓝染色切片中很明显，在组织选择(商标)(R)固定组织中糖胺多糖的丢失是显著的。然而，组织选择固定在聚集素和聚集素免疫组织学应用中效果良好，在这些应用中，其非交联性特征有助于通过细胞外基质成分的一次抗体检索和鉴定表位。
Histochoice (TM)(R) is a proprietary nontoxic, non-cross-linking fixative designed by the manufacturer to replace formaldehyde based fixation protocols. We compared Histochoice and formalin fixation for several cartilaginous tissues including, articular and growth plate cartilage, meniscus and intervertebral disc. The tissues were stained with general histology stains including toluidine blue for tissue proteoglycans, picrosirius red to evaluate collagenous organization, and hematoxylin and eosin to assess cell morphology. The chondroitin sulfate and heparin sulfate substituted proteoglycans aggrecan and perlecan were also immunolocalized in some of the tissues to provide a comparison. Histochoice did not fix deep into the tissue blocks resulting in focal loss of aggrecan and other matrix components from the more central regions of the blocks. This was evident in toluidine blue stained sections of immature tibial articular cartilage where loss of glycosaminoglycan was significant in Histochoice (TM)(R) fixed tissues. Histochoice fixation worked well, however, in the aggrecan and perlecan immunohistology applications where its non-cross-linking traits were conducive to epitope retrieval and identification by primary antibodies to extracellular matrix components.