Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus plantarum 70810
Structural characterization and bioactivity of released exopolysaccharides from Lactobacillus plantarum 70810
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植物乳杆菌 70810 释放的胞外多糖的结构表征和生物活性
DOI：
10.1016/j.ijbiomac.2014.02.056
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发表时间：
2014-06-01
影响因子：
8.2
通讯作者：
Dong, Mingsheng
中科院分区：
文献类型：
--
作者：
Wang, Kun;Li, Wei;Dong, Mingsheng
采用乙醇沉淀、阴离子交换和凝胶过滤层析等方法从植物乳杆菌70810的培养上清液中分离得到两种胞外多糖（r-EPS 1和r-EPS 2），并对其结构进行了表征。r-EPS 1和r-EPS 2均为均一多糖，平均分子量分别为204.6和202.8 kDa，由葡萄糖、甘露糖和半乳糖组成，摩尔比分别为18.21：78.76：3.03和12.92：30.89：56.19。甲基化和NMR分析表明r-EPS 1由α构型糖残基组成，而r-EPS 2含有α和β构型糖残基。体外抗氧化活性和抗肿瘤活性进行了评价。结果表明，r-EPS 1和r-EPS 2具有较强的羟自由基和DPPH自由基清除能力和还原能力，并对Caco-2、BGC-823和HT-29细胞具有一定的抗肿瘤活性，有望成为抗氧化和抗肿瘤药物的潜在来源。(C)2014爱思唯尔有限公司版权所有。
Two released exopolysaccharides (r-EPS1 and r-EPS2) were isolated from the culture supernatant of Lactobacillus plantarum 70810 by ethanol precipitation, anion exchange and gel filtration chromatography and characterization of the structures were conducted. The r-EPS1 and r-EPS2 were homogenous polysaccharides with average molecular weights of, respectively, 204.6 and 202.8 kDa, and composed of glucose, mannose and galactose with molar ratios of 18.21:78.76:3.03 and 12.92:30.89:56.19, respectively. Methylation and NMR analysis revealed r-EPS1 was consisted of alpha configuration sugar residues, whereas r-EPS2 contained both alpha and beta configuration sugar residues. Antioxidant and antitumor activities of the two exopolysaccharides were evaluated in vitro. The results showed that r-EPS1 and r-EPS2 exhibited potent antioxidant activities regarding hydroxyl and DPPH radicals scavenging and reducing power assays, and possessed antitumor activities against Caco-2, BGC-823 and HT-29 cells, which suggested that they could be potential sources of antioxidant and antitumor drug. (C) 2014 Elsevier B.V. All rights reserved.