Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins
Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins
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DOI：
10.1093/nar/29.11.2205
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发表时间：
2001-06-01
影响因子：
14.9
通讯作者：
Hatfull, GF
中科院分区：
文献类型：
--
作者：
Lewis, JA;Hatfull, GF
DNA底物和整合酶介导的位点特异性重组反应产物之间的相似性导致能够催化整合和切除反应的单一重组酶。在这些反应中，方向性的控制是通过一类小的辅助因素来实现的，这些辅助因素有利于一个反应，同时干扰另一个反应。这些蛋白质，我们将统称为重组方向性因子（RDF），在由其同源重组酶催化的反应中发挥结构作用，并已与酪氨酸和丝氨酸整合酶一起鉴定。以前鉴定的RDF通常是小的，碱性的，并具有不同的氨基酸序列。RDF的一个子集，考克斯基因，也作为转录调节因子。我们在这里提出了一个汇编的所有已知的RDF蛋白质，以及那些通过数据库挖掘，我们预测将参与赋予重组方向性，这组蛋白质的分析表明，它们可以被分组为不同的亚组的基础上，他们的序列相似性，他们很可能已经出现了几个独立的进化谱系。这种编译将证明是有用的，在识别新的蛋白质，赋予定向后，位点特异性重组反应编码的质粒，转座子，噬菌体和原噬菌体。
Similarity between the DNA substrates and products of integrase-mediated site-specific recombination reactions results in a single recombinase enzyme being able to catalyze both the integration and excision reactions. The control of directionality in these reactions is achieved through a class of small accessory factors that favor one reaction while interfering with the other. These proteins, which we will refer to collectively as recombination directionality factors (RDFs), play architectural roles in reactions catalyzed by their cognate recombinases and have been identified in conjunction with both tyrosine and serine integrases, Previously identified RDFs are typically small, basic and have diverse amino acid sequences, A subset of RDFs, the cox genes, also function as transcriptional regulators. We present here a compilation of all the known RDF proteins as well as those identified through database mining that we predict to be involved in conferring recombination directionality, Analysis of this group of proteins shows that they can be grouped into distinct subgroups based on their sequence similarities and that they are likely to have arisen from several independent evolutionary lineages. This compilation will prove useful in recognizing new proteins that confer directionality upon site-specific recombination reactions encoded by plasmids, transposons, phages and prophages.