Eimeria tenella:: Cloning of a novel Eimeria tenella cDNA encoding a protein related to rhomboid family from F2 hybrid strain
Eimeria tenella:: Cloning of a novel Eimeria tenella cDNA encoding a protein related to rhomboid family from F2 hybrid strain
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DOI：
10.1016/j.exppara.2006.01.011
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发表时间：
2006-08-01
影响因子：
2.1
通讯作者：
Yang, Ju
中科院分区：
文献类型：
--
作者：
Li, Jianhua;Zhang, Xichen;Yang, Ju
用抗子孢子单克隆抗体从λ cDNA文库中克隆到柔嫩艾美耳球虫（Eimeria tenella）F2杂交株（ETRH 0 1）的一个新cDNA序列，其开放阅读框为774 bp。基因组序列分析表明这是一个无内含子基因。推导的蛋白质序列由257个氨基酸组成，计算分子量为28.349 kDa，等电点为8.56。序列分析表明，七个跨膜结构域和菱形结构域的蛋白质。RT-PCR结果表明，该基因在5株E. tenella分离物分析。为了进一步研究该基因在大肠杆菌生活史中的作用。tenella，利用pET 28 b（+）表达系统成功表达ETRH 01。(c)2006爱思唯尔公司All rights reserved.
A novel cDNA sequence with an open reading frame of 774 bp from Eimeria tenella F2 hybrid strain (ETRH01) was isolated from a lamda cDNA library with a monoclonal antibody against sporozoite. Analysis of the genomic sequence suggests that this is an intronless gene. The deduced protein sequence has 257 amino acids with a calculated molecular weight of 28.349 kDa and an isoelectric point of 8.56. Sequence analysis revealed seven transmembrane domains and a rhomboid domain within the protein. RT-PCR result indicates that this gene was expressed in all of the five E. tenella isolates analyzed. To further study the role of this novel gene in the life cycle of E. tenella, ETRH01 was successfully expressed using pET28b(+) expression system. (c) 2006 Elsevier Inc. All rights reserved.