Quorum-sensing and cheating in bacterial biofilms.
Quorum-sensing and cheating in bacterial biofilms.
复制标题
细菌生物膜中的法定感应和作弊。
DOI：
10.1098/rspb.2012.1976
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发表时间：
2012-12-07
期刊：
影响因子：
--
通讯作者：
Diggle SP
中科院分区：
文献类型：
--
作者：
Popat R;Crusz SA;Messina M;Williams P;West SA;Diggle SP
人类社会认为，自利可能导致群体层面合作的破裂，这一观点有时被称为公共产品困境。我们在机会致病菌铜绿假单胞菌中测试了这一想法，通过检查不通过细胞间信号传导（群体感应，QS）合作的推定作弊的影响。我们发现：（i）由于利用QS调节的公共物品，QS欺骗发生在生物膜种群中;（ii）非合作欺骗的存在降低了生物膜的厚度和密度;（iii）欺骗的存在降低了种群增长，并且这种降低在生物膜中比在非合作种群中更大;（iv）欺骗的存在增加了生物膜对抗生素的敏感性;以及（v）强迫合作者细胞增加它们的合作水平降低了作弊的存在降低群体生产力的程度。我们的研究结果提供了明确的支持，公共产品的冲突减少了细菌生物膜中的人口适应度，这种影响大于在无菌人群中。最后，我们讨论了改变对抗生素的敏感性所产生的临床意义。
The idea from human societies that self-interest can lead to a breakdown of cooperation at the group level is sometimes termed the public goods dilemma. We tested this idea in the opportunistic bacterial pathogen, Pseudomonas aeruginosa, by examining the influence of putative cheats that do not cooperate via cell-to-cell signalling (quorum-sensing, QS). We found that: (i) QS cheating occurs in biofilm populations owing to exploitation of QS-regulated public goods; (ii) the thickness and density of biofilms was reduced by the presence of non-cooperative cheats; (iii) population growth was reduced by the presence of cheats, and this reduction was greater in biofilms than in planktonic populations; (iv) the susceptibility of biofilms to antibiotics was increased by the presence of cheats; and (v) coercing cooperator cells to increase their level of cooperation decreases the extent to which the presence of cheats reduces population productivity. Our results provide clear support that conflict over public goods reduces population fitness in bacterial biofilms, and that this effect is greater than in planktonic populations. Finally, we discuss the clinical implications that arise from altering the susceptibility to antibiotics.
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影响因子：
3.2
作者：
Heeb, S;Blumer, C;Haas, D
通讯作者：
Haas, D
DOI：
10.1098/rsbl.2003.0083
发表时间：
2004-02-07
影响因子：
4.7
作者：
Greig, D;Travisano, M
通讯作者：
Travisano, M
DOI：
10.1098/rspb.2003.2387
发表时间：
2003-07-22
影响因子：
4.7
作者：
Fiegna, F;Velicer, GJ
通讯作者：
Velicer, GJ
影响因子：
2.1
作者：
Jiricny, N.;Diggle, S. P.;Griffin, A. S.
通讯作者：
Griffin, A. S.
DOI：
10.1007/s10295-006-0086-3
发表时间：
2007-01-01
影响因子：
3.4
作者：
Burton, E.;Yakandawala, N.;Madhyastha, M. S.
通讯作者：
Madhyastha, M. S.