Phytochemical investigation of Tunisian Salicornia herbacea L., antioxidant, antimicrobial and cytochrome P450 (CYPs) inhibitory activities of its methanol extract
Phytochemical investigation of Tunisian Salicornia herbacea L., antioxidant, antimicrobial and cytochrome P450 (CYPs) inhibitory activities of its methanol extract
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DOI：
10.1016/j.foodcont.2012.11.006
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发表时间：
2013-07-01
期刊：
影响因子：
6
通讯作者：
Bouzouita, Nabiha
中科院分区：
文献类型：
--
作者：
Essaidi, Ismahen;Brahmi, Zeineb;Bouzouita, Nabiha
本文研究了海蓬子的化学成分。摘要对藜科一种食用植物的矿物质组成、蛋白质和碳水化合物含量进行了研究。高效液相色谱二极管阵列检测器（HPLC-DAD）分析的酚类成分显示存在8个酚酸和8个黄酮类化合物。采用1，1-二苯基-2-苦基肼（DPPH）自由基清除试验和β-胡萝卜素亚油酸漂白试验对甲醇提取物的抗氧化活性进行评价。该提取物是一种有效的自由基清除剂（IC 50 = 55.3 +/- 2.7 μ g/mL），并表现出中度抑制亚油酸乳剂的脂质过氧化作用。用纸片扩散法测定了该化合物对4种革兰氏阴性菌和2种革兰氏阳性菌的抑菌活性，结果表明该化合物对所有菌株均有抑菌作用，并首次测定了该化合物对细胞色素P450（CYP）3种同工酶（CYP 1A 2、CYP 3A 4和CYP 2D 6）的抑制活性。该提取物是CYP 2D 6的强效抑制剂（IC 50 = 3.88 +/- 0.02 μ g/mL）。(C)2012爱思唯尔有限公司保留所有权利。
The chemical composition of Salicornia herbacea L. a food plant belonging to Chenopodiaceae was investigated based on its mineral composition, proteins and carbohydrates contents. The phenolic composition analyzed by high performance liquid chromatography with diode array detection (HPLC-DAD) showed the presence of eight phenolic acids and eight flavonoids. The biological activities of the methanol extract were conducted on the antioxidant activity evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and beta-carotene linoleic acid bleaching test. The extract was an efficient radical scavenger (IC50 = 55.3 +/- 2.7 mu g/mL) and exhibited a moderate inhibition of lipid peroxidation of linoleic acid emulsion. Antibacterial activity determined by disc diffusion method against four Gram negative and two Gram positive bacteria, showed effects against all strains.Cytochrome P450s (CYPs) inhibitory activity studied for the first time was evaluated against three CYP isozymes namely CYP1A2, CYP3A4 and CYP2D6. The extract was a potent inhibitor against CYP2D6 (IC50 = 3.88 +/- 0.02 mu g/mL). (C) 2012 Elsevier Ltd. All rights reserved.