Purification of the Staphylococcus aureus bacteriophages VDX-10 on methacrylate monoliths
Purification of the Staphylococcus aureus bacteriophages VDX-10 on methacrylate monoliths
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DOI：
10.1016/j.jviromet.2010.02.020
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发表时间：
2010-06-01
影响因子：
3.1
通讯作者：
Peterka, Matjaz
中科院分区：
文献类型：
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作者：
Kramberger, Petra;Honour, Richard C.;Peterka, Matjaz
已知噬菌体（Bacteriophages，简称BPHs）可用于从医学到农业的许多领域，并且用于广泛的应用，包括噬菌体治疗和噬菌体展示。对于某些应用，特别是在医学中，需要高纯度和高活力的噬菌体。将设计用于纯化生物纳米颗粒的甲基丙烯酸酯整料（对流相互作用介质[CIM]整料柱）应用于从细菌裂解物中纯化金黄色葡萄球菌VDX-10。通过一步纯化方法，去除了99%以上的宿主细胞DNA和90%以上的蛋白质，活菌体的回收率为60%。当纯化方法从CIM整体盘放大到更大的CIM整体柱时，获得了可比的结果。此外，甲基丙烯酸酯整体柱对S。测定了金黄色葡萄球菌VDX-10。(C)2010爱思唯尔有限公司版权所有。
Bacteriophages (phages) are known to be useful in many fields from medicine to agriculture, and for a broad range of applications, including phage therapy and phage display. For some applications, especially in medicine, high purity and viability of phages are required. Methacrylate monoliths (Convective Interaction Media [CIM] monolithic columns), designed for purification of bionanoparticles, were applied for the purification of Staphylococcus aureus phages VDX-10 from bacterial lysate. With a single step purification method, more than 99% of host cell DNA and more than 90% of proteins were removed, with 60% recovery of viable phages. Comparable results were obtained when the purification method was scaledup from a CIM monolithic disk to a larger CIM monolithic column. Additionally, the dynamic binding capacity of a methacrylate monolith column for S. aureus phages VDX-10 was determined. (C) 2010 Elsevier B.V. All rights reserved.