BASE SEQUENCE STUDIES OF 300 NUCLEOTIDE RENATURED REPEATED HUMAN DNA CLONES
BASE SEQUENCE STUDIES OF 300 NUCLEOTIDE RENATURED REPEATED HUMAN DNA CLONES
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DOI：
10.1016/0022-2836(81)90219-9
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
SCHMID, CW
中科院分区：
文献类型：
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作者：
DEININGER, PL;JOLLY, DJ;SCHMID, CW
通过用单链特异性核酸内切酶S1处理复性的重复人DNA，释放出300个核苷酸长的双链DNA条带。由于人类DNA中许多散布的重复序列是300个核苷酸长，因此该条带应该富含这样的重复序列。从这300个核苷酸的S1-抗性重复序列构建的15个克隆的核苷酸序列进行了测定。这些克隆序列中的10个是Alu家族的散置重复序列的成员。这10个序列共享一个可识别的共有序列，其中单个克隆的平均差异为12.8%。300个核苷酸的Alu家族共有序列具有二聚体结构，并且显然是由祖先单体序列的首尾重复形成的。其余三个克隆是之前报道的人类卫星III DNA简单五核苷酸序列的变体。15个克隆中的两个具有不同且复杂的序列，并且可能代表散布的重复序列的其他家族。
A band of 300 nucleotide long duplex DNA is released by treating renatured repeated human DNA with the single strand-specific endonuclease S1. Since many of the interspersed repeated sequences in human DNA are 300 nucleotides long, this band should be enriched in such repeats. The nucleotide sequences of 15 clones constructed from these 300 nucleotide S1-resistant repeats were determined. Ten of these cloned sequences are members of the Alu family of interspersed repeats. These 10 sequences share a recognizable consensus sequence from which individual clones have an average divergence of 12.8%. The 300 nucleotide Alu family consensus sequence has a dimeric structure and was evidently formed from a head to tail duplication of an ancestral monomeric sequence. Three of the remaining clones are variations on a simple pentanucleotide sequence previously reported for human satellite III DNA. Two of the 15 clones have distinct and complex sequences and may represent other families of interspersed repeated sequences.