Germ-line transformation and RNAi of the ladybird beetle, Harmonia axyridis
Germ-line transformation and RNAi of the ladybird beetle, Harmonia axyridis
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DOI：
10.1111/j.1365-2583.2006.00665.x
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发表时间：
2006-08-01
影响因子：
2.6
通讯作者：
Niimi, T.
中科院分区：
文献类型：
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作者：
Kuwayama, H.;Yaginuma, T.;Niimi, T.
为了阐明昆虫翅膀颜色模式的巨大多样性的分子机制，必须确定和功能特性的基因参与这一发展过程。在这里，我们报告的第一个成功的生殖系转化使用转座因子载体piggyBac在瓢虫异色瓢虫，这表明其翅膀的颜色模式的典型遗传多态性。piggyBac对每个可育G（0）的转化效率为3.7%。我们通过将EGFP（增强型绿色荧光蛋白）dsRNA注射到表达EGFP的早期转基因胚胎中来研究RNAi在Harmonia中的有效性，并观察到在87.2%的孵化幼虫中EGFP荧光显著降低。将这些新的遗传工具应用于非模式昆虫，如Harmonia，将有助于广泛了解使用已建立的模型系统无法访问的发育机制和进化过程。
To elucidate the molecular mechanisms underlying the tremendous diversity of insect wing colour patterns, it is imperative to identify and functionally characterize the genes involved in this developmental process. Here we report the first successful germ-line transformation using the transposable element vector piggyBac in the ladybird beetle Harmonia axyridis, which demonstrates typical genetic polymorphism in its wing colour patterns. The transformation efficiency by piggyBac was 3.7% per fertile G(0). We investigated the effectiveness of RNAi in Harmonia by injecting EGFP (enhanced green fluorescent protein) dsRNA into early transgenic EGFP-expressing embryos and observed substantial reduction of EGFP fluorescence in 87.2% of hatched larvae. Application of these new genetic tools to non-model insects such as Harmonia will facilitate the broad understanding of developmental mechanisms and evolutionary processes that are inaccessible using established model systems.