[Effects of electromagnetic noise on the enhancement of stress-activated protein kinase(SAPK) phosphorylation induced by 50 Hz magnetic fields].
[Effects of electromagnetic noise on the enhancement of stress-activated protein kinase(SAPK) phosphorylation induced by 50 Hz magnetic fields].
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发表时间：
2002-08
影响因子：
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通讯作者：
Wenjun Sun;H. Chiang;Yi-ti Fu;D. Lu;Zheng-ping Xu
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文献类型：
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作者：
Wenjun Sun;H. Chiang;Yi-ti Fu;D. Lu;Zheng-ping Xu
目的探讨电磁噪声暴露对50 Hz磁场诱导的应激激活蛋白激酶（SAPK）磷酸化的可能干扰作用。方法将中国仓鼠肺（CHL）细胞分别暴露于假暴露（C）、0.4mT 50Hz正弦磁场、0.4mT电磁噪声以及磁场与50Hz磁场联合作用3min和15min。暴露后，裂解细胞，并提取蛋白质。SAPK和磷酸化的SAPK（活化形式的SAPK）用相应的抗体通过Western印迹间接测量。计算并分析磷酸化SAPK的百分比。结果50 Hz磁场作用3 min和15 min可使SAPK磷酸化增强。磷酸化SAPK的比例分别为49.3%和57.0%，与对照组相比差异有显著性（P0.05）。当噪声MF与50Hz MF联合作用3min时，SAPK磷酸化水平明显降低（24.4%，P0.05）。结论一定强度的噪声磁场可抑制50Hz磁场的生物学效应。
OBJECTIVE To investigate the possible interference effect of electromagnetic noise exposure on phosphorylation of stress-activated protein kinase(SAPK) induced by 50 Hz magnetic field(MF). METHODS Chinese hamster lung(CHL) cells were exposed to sham exposure(C), 0.4 mT 50 Hz sinusoidal MF, 0.4 mT electromagnetic noise and the combined noise MF with 50 Hz MF for 3 min and 15 min respectively. After exposure, the cells were lysed, and the proteins were extracted. The SAPK and phosphorylated SAPK (activated form of SAPK) were measured indirectly by Western blot with corresponding antibodies. The percentage of phosphorylated SAPK was calculated and analyzed. RESULTS Exposure of cells to 50 Hz MF for 3 min and 15 min enhanced the SAPK phosphorylation. The percentage of phosphorylated SAPK were 49.3% and 57.0% respectively, and were significantly different from those of control(P 0.05). When cells were exposed to the combined noise MF with 50 Hz MF for 3 min, the SAPK phosphorylation was significantly inhibited (24.4%, P 0.05). CONCLUSION Noise MF with certain intensity could inhibit the biological effect induced by 50 Hz MF.