Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization
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DOI：
10.1039/c5an02620b
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发表时间：
2016-01-01
期刊：
影响因子：
4.2
通讯作者：
Lanekoff, Ingela
中科院分区：
文献类型：
--
作者：
Bergman, Hilde-Marlene;Lundin, Erik;Lanekoff, Ingela
小分子神经递质对神经系统的功能至关重要，神经递质失衡通常与神经系统疾病有关。量化这种不平衡的能力对于深入了解这种疾病的生化机制非常重要。这项原理验证研究采用纳米喷雾解吸电喷雾电离（nano-DESI）质谱成像，在线定量大鼠脑组织切片中的小分子神经递质，特别是乙酰胆碱、γ-氨基丁酸（GABA）和谷氨酸。通过在纳米DESI溶剂中掺入氘代内标，我们显示了这些小神经递质在大鼠脑组织中的识别、准确映射和定量，而无需引入任何额外的样品制备步骤。我们发现GABA在内侧隔-斜角带复合体（MSDB）中的丰度约为皮质中的两倍，而谷氨酸在皮质中的丰度约为MSDB的两倍。该研究表明，纳米DESI非常适合健康和疾病中小分子神经递质的成像。
Small molecule neurotransmitters are essential for the function of the nervous system, and neurotransmitter imbalances are often connected to neurological disorders. The ability to quantify such imbalances is important to provide insights into the biochemical mechanisms underlying the disorder. This proof-of-principle study presents online quantification of small molecule neurotransmitters, specifically acetylcholine, gamma-aminobutyric acid (GABA) and glutamate, in rat brain tissue sections using nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging. By incorporating deuterated internal standards in the nano-DESI solvent we show identification, accurate mapping, and quantification of these small neurotransmitters in rat brain tissue without introducing any additional sample preparation steps. We find that GABA is about twice as abundant in the medial septum-diagonal band complex (MSDB) as in the cortex, while glutamate is about twice as abundant in the cortex as compared to the MSDB. The study shows that nano-DESI is well suited for imaging of small molecule neurotransmitters in health and disease.