Miniaturized Electroosmotic Pump Capable of Generating Pressures of More than 1200 Bar
Miniaturized Electroosmotic Pump Capable of Generating Pressures of More than 1200 Bar
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DOI：
10.1021/ac3025703
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发表时间：
2012-11-06
影响因子：
7.4
通讯作者：
Liu, Shaorong
中科院分区：
文献类型：
--
作者：
Gu, Congying;Jia, Zhijian;Liu, Shaorong
泵的压力输出不能简单地通过串联几个泵来增加。本文所研制的微泵消除了这一障碍。该泵实际上是由多个串联的基本泵单元组成的总成。这种泵组件的最大压力输出与串联的泵单元的数量成正比。从理论上讲，人们总是可以通过在组件中增加更多的泵单元来提高压力输出，但实际上，上限压力受到连接泵部件的微三通或微接头的限制。使用商用的微三通和微接头，压力已达到1200巴以上。我们最近试验了使用开放式毛细管来制造这种泵，但必须并行使用许多毛细管才能产生足够的流量来驱动高效液相色谱分离。在本论文中，我们合成了直径为75微米的聚合物整体。毛细管，使用这些整体组装微型泵，表征这些泵的性能，并使用这些泵对完整蛋白质进行高效液相色谱分离。通过调整整体柱制备的实验参数，可以方便地获得带正负电荷的亚微米级毛细管通道。每一块都在75亩长的直径内。毛细血管相当于数千条开放的毛细血管。
The pressure output of a pump cannot be increased simply by connecting several of them in series. This barrier is eliminated with the micropump developed in this work. The pump is actually an assembly of a number of fundamental pump units connected in series. The maximum pressure output of this pump assembly is directly proportional to the number of serially connected pump units. Theoretically, one can always enhance the pressure output by adding more pump units in the assembly, but in reality the upper pressure is constrained by the microtees or microunions joining the pump components. With commercially available microtees and microunions, pressures of more than 1200 bar have been achieved. We have recently experimented using open capillaries to build this pump, but many capillaries have to be utilized in parallel to produce an adequate flow to drive HPLC separations. In this paper, we synthesize polymer monoliths inside 75 mu m id. capillaries, use these monoliths to assemble miniaturized pumps, characterize the performance of these pumps, and employ these pumps for HPLC separations of intact proteins. By tuning the experimental parameters for monolith preparations, we obtain both negatively and positively charged submicrometer capillary channels conveniently. Each monolith in a 75 mu m id. capillary is equivalent to several thousands of open capillaries.