Recommended minimal standards for description of new staphylococcal species
Recommended minimal standards for description of new staphylococcal species
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DOI：
10.1099/00207713-49-2-489
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发表时间：
1999-04-01
期刊：
影响因子：
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通讯作者：
Vernozy-Rozand, C
中科院分区：
文献类型：
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作者：
Freney, J;Kloos, WE;Vernozy-Rozand, C
根据《国际细菌命名法典》建议 30b，提出了葡萄球菌属的最低标准以及该属中新识别物种的描述。将菌株归为葡萄球菌属要求它是革兰氏阳性球菌，能够形成簇、产生过氧化氢酶、具有适当的细胞壁结构（包括肽聚糖类型和磷壁酸的存在）以及DNA的G+C含量在30-40mol%的范围内。描述新葡萄球菌物种的推荐最低标准基于至少五种独立分离菌株的表型和基因组研究结果。它们包括菌落形态和以下常规测试的结果：色素产生、生长要求、碳水化合物的发酵和氧化活性、新生霉素敏感性、酶活性（硝酸还原酶、碱性磷酸酶、精氨酸二水解酶、鸟氨酸脱羧酶、脲酶、细胞色素氧化酶、葡萄球菌凝固酶） 血浆、热稳定性核酸酶、酰胺酶、氧化酶、凝集因子以及羊或牛血琼脂上的溶血活性）。当同源反应中的结合与异源反应中的结合之间以百分比表示的差异小于70％时，DNA-DNA杂交实验可以区分物种。此外，建议使用 rRNA 特征序列标准、命名类型菌株和该物种的其他菌株的核糖分型特征以及其他物种的参考菌株来描述具有遗传属性集的新物种菌株并揭示可能的分组错误。该提案得到了国际系统细菌学委员会葡萄球菌和链球菌分类学小组委员会成员的认可。
In accordance with Recommendation 30b of the International Code of Nomenclature of Bacteria, minimal standards are proposed for the genus Staphylococcus and the description of newly recognized species in this genus. Assignment of a strain to the genus Staphylococcus requires that it is a Grampositive coccus that forms clusters, produces catalase, has an appropriate cell wall structure (including peptidoglycan type and teichoic acid presence) and G+C content of DNA in a range of 30-40 mol%. The recommended minimal standards for describing a new Staphylococcus species are based on the results of phenotypic and genomic studies of at least five independently isolated strains. They include colony morphology and the results of the following conventional tests: pigment production, growth requirements, fermentative and oxidative activity on carbohydrates, novobiocin susceptibility, enzymic activities (nitrate reductase, alkaline phosphatase, arginine dihydrolase, ornithine decarboxylase, urease, cytochrome oxidase, staphylocoagulase in rabbit plasma, heat-stable nuclease, amidases, oxidases, clumping factor, and haemolytic activity on sheep or bovine blood agar). DNA-DNA hybridization experiments may distinguish species when the difference between the binding in the homologous reaction and the binding in the heterologous reaction expressed as a percentage is less than 70%. In addition, rRNA signature sequence criteria, ribotyping characterization of the nomenclature type strain and other strains of the species, and reference strains of other species is recommended to describe the strains of the new species with sets of genetic attributes and reveal possible grouping errors. This proposal has been endorsed by the members of the Subcommittee on the taxonomy of staphylococci and streptococci of the International Committee on Systematic Bacteriology.