Trehalose promotes the formation of Lanzhou lily bulblets by increasing carbohydrate content
Trehalose promotes the formation of Lanzhou lily bulblets by increasing carbohydrate content
复制标题
海藻糖通过增加碳水化合物含量促进兰州百合鳞茎的形成
DOI：
10.1080/14620316.2021.2023331
复制
发表时间：
2022-01-08
影响因子：
1.9
通讯作者：
Liao, Weibiao
中科院分区：
文献类型：
--
作者：
Wu, Xuetong;Li, Weitai;Liao, Weibiao
摘要：最近的研究表明，海藻糖在植物中发挥着重要而普遍的作用，它积极调节种子发芽、开花和抗逆性。但海藻糖是否促进兰州百合鳞片扦插繁殖中小鳞茎的形成尚未见报道。在当前的研究中，研究了海藻糖在百合鳞茎形成过程中的调节作用。结果表明，海藻糖显着增加了鳞茎的数量和直径，最佳浓度为50 mM。此外，海藻糖还增加了鳞片和鳞茎中蔗糖、淀粉、可溶性糖和内源海藻糖的含量。这些结果表明，海藻糖可能通过增加鳞片中碳水化合物的含量来促进兰州百合鳞茎的形成。此外，海藻糖提高了海藻糖6-磷酸合酶（TPS）和ADP葡萄糖焦磷酸化酶（AGP）活性，降低了鳞片中的蔗糖合酶（SuSy）活性，上调了第50天鳞片中SSS1、SBE、AGP1、TPS6、TPS7、TPS9的表达水平，并下调了鳞片中SuSy1和INV2的表达水平。因此，海藻糖可能通过调节碳水化合物合成和水解途径中关键酶的活性和基因表达，增加鳞片和鳞茎中碳水化合物的含量，从而促进兰州百合鳞茎插条中鳞茎的形成。
ABSTRACT Recent studies have shown that trehalose plays an important and pervasive role in plants, which actively regulates seed germination, flowering, and stress tolerance. However, whether trehalose promotes bulblet formation in Lanzhou lily (Lilium davidii) scale cutting propagation has not been reported. In the current study, the regulatory roles of trehalose during the formation of bulblets in lily-scale cutting were investigated. The results show that trehalose significantly increased the number and diameter of bulblets with an optimal concentration at 50 mM. Moreover, trehalose increased the content of sucrose, starch, soluble sugar and endogenous trehalose in the scales and bulblets. These results indicate that trehalose might promote the formation of Lanzhou lily bulblets by increasing the carbohydrate content in the scales. Further, trehalose improved trehalose 6-phosphate synthase (TPS) and ADP glucose pyrophosphorylase (AGP) activity, reduced Sucrose synthase (SuSy) activity in scales, up-regulated SSS1, SBE, AGP1, TPS6, TPS7, TPS9 expression level in scales on day 50, and down-regulated SuSy1 and INV2 expression level in scales. Therefore, trehalose might increase carbohydrates content in scales and bulblets by regulating the activity and gene expression of key enzymes in carbohydrate synthesis and hydrolysis pathways, thereby promoting bulblet formation in Lanzhou lily-scale cutting.