Genotypes of canine distemper virus determined by analysis of the hemagglutinin genes of recent isolates from dogs in Japan
Genotypes of canine distemper virus determined by analysis of the hemagglutinin genes of recent isolates from dogs in Japan
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DOI：
10.1128/jcm.37.9.2936-2942.1999
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发表时间：
1999-09-01
影响因子：
9.4
通讯作者：
Ishiguro, S
中科院分区：
文献类型：
--
作者：
Mochizuki, M;Hashimoto, M;Ishiguro, S
家犬的犬瘟热是由犬瘟热病毒（CDV）引起的，CDV是麻疹病毒的一种。几个世纪以来，它一直是一种高度传染性的疾病，对兽医具有重要意义，但在过去的几十年里，它已被改良的活疫苗控制得令人满意。然而，在20世纪90年代，有人描述说，CDV毒株在遗传上与疫苗毒株不同，可能导致接种疫苗的狗患病。最高的抗原变异在H蛋白中发现。因此，在本研究中，通过限制性片段长度多态性分析和测序对从当前疫苗和保持分离株中获得并直接从临床标本中扩增的血凝素（H）基因进行遗传分析。ii-基因氨基酸序列的系统发育分析显示，至少有两种CDV基因型在日本的犬中传播;一种是几乎所有日本CDV分离株所属的基因型，另一种以前没有描述过。两者均与疫苗株的其他谱系或基因型以及欧洲和美国CDV分离株分开且独立。结果表明，CDV在日本也发生了演变，需要进一步研究以评估和可能改善目前CDV疫苗的效力。
Canine distemper of domestic dogs is caused by canine distemper virus (CDV), a member of the morbilliviruses. It has been a highly contagious disease of great veterinary importance for centuries, but for the last several decades it has been controlled satisfactorily by modified live vaccines. In the 1990s, however, it was described that CDV strains genetically different from vaccine strains may have caused the disease in vaccinated dogs. The highest antigenic variation is found in the H protein. Therefore, in the present study, hemagglutinin (H) genes obtained from current vaccines and held isolates and amplified directly from clinical specimens were genetically analyzed by restriction fragment length polymorphism assay and sequencing. Phylogenetic analysis of the ii-gene amino acid sequences revealed that at least two CDV genotypes are circulating among dogs in Japan; one is a genotype to which almost all Japanese CDV isolates belong and the other has not been previously described. Both are separate and independent from the other lineages or genotypes of vaccine strains, as well as European and U.S. CDV isolates. The results suggest that CDV has also evolved in Japan, and further studies will be needed for an evaluation and possible improvement of the efficacies of current CDV vaccines.