Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.
Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.
复制标题
α病毒RNA复制酶位于内体和溶酶体的细胞质表面上。
DOI：
10.1083/jcb.107.6.2075
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发表时间：
1988-12
影响因子：
7.8
通讯作者：
Helenius, A
中科院分区：
文献类型：
--
作者：
Froshauer, S;Kartenbeck, J;Helenius, A
利用形态学和细胞生物学技术，我们发现Semliki Forest病毒和Sindbis病毒（两种密切相关的甲病毒）的RNA复制酶位于与修饰的次级溶酶体和核内体细胞质表面相关的复杂核糖核蛋白结构中。这些核蛋白复合物通常在内吞液泡膜和粗内质网之间形成桥梁，在粗内质网中发生这些病毒结构蛋白的合成。结果表明，这些细胞病变液泡不仅是病毒RNA合成的位点，也是结构蛋白翻译和核衣壳组装的位点。
Using morphological and cell biological techniques, we have shown that the RNA replicase of Semliki Forest and Sindbis virus (two closely related alphaviruses) is located in complex ribonucleoprotein structures associated with the cytoplasmic surface of modified secondary lysosomes and endosomes. These nucleoprotein complexes often form a bridge between the membrane of the endocytic vacuole and the rough endoplasmic reticulum where the synthesis of the structural proteins of these viruses occurs. The results suggest that these cytopathic vacuoles constitute sites not only for viral RNA synthesis, but also for translation of structural proteins, and for the assembly of nucleocapsids.