The meta and bioinformatics analysis of GRP78 expression in gastric cancer.
The meta and bioinformatics analysis of GRP78 expression in gastric cancer.
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胃癌中GRP78表达的Meta及生物信息学分析
DOI：
10.18632/oncotarget.20318
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发表时间：
2017-09-22
期刊：
影响因子：
--
通讯作者：
Zhao S
中科院分区：
文献类型：
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作者：
Zheng HC;Gong BC;Zhao S
GRP78是一种位于内质网的分子伴侣，可诱导新合成蛋白的折叠和组装、异常蛋白的蛋白酶体降解、分泌蛋白的易位、自噬和上皮-间质转化。截至2017年3月14日，我们通过多个在线数据库进行了系统的元和生物信息学分析。结果发现，胃癌组织中GRP78蛋白和mRNA水平均较正常粘膜上调（p < 0.05）。GRP78 mRNA表达与胃癌浸润深度、TNM分期、去分化呈显著正相关（p < 0.05），与胃癌浸润深度、组织学分级、去分化呈显著负相关（p < 0.05）。胃癌患者GRP78表达与不良总生存率呈正相关（p < 0.005）。GRP78 mRNA的高表达与所有癌症患者的总体生存率和无进展生存率呈正相关，甚至根据侵袭性参数分层，或作为一个独立因素（p < 0.05）。这些发现提示GRP78的表达可能作为胃癌发生及其后续进展甚至预后的潜在标志物。
GRP78 is a molecular chaperone located in endoplasmic reticulum, and induces folding and assembly of newly-synthesized proteins, proteasome degradation of aberrant proteins, and translocation of secretory proteins, autophagy, and epithelial-mesenchymal transition. We performed a systematic meta- and bioinformatics analysis through multiple online databases up to March 14, 2017. It was found that up-regulated GRP78 expression in gastric cancer, compared with normal mucosa at both protein and mRNA levels (p < 0.05). GRP78 expression was positively correlated with depth of invasion, TNM staging and dedifferentiation of gastric cancer (p < 0.05), while its mRNA expression was negatively correlated with depth of invasion, histological grading and dedifferentiation (p < 0.05). A positive association between GRP78 expression and unfavorable overall survival was found in patients with gastric cancer (p < 0.005). A higher GRP78 mRNA expression was positively correlated with overall and progression-free survival rates of all cancer patients, even stratified by aggressive parameters, or as an independent factor (p < 0.05). These findings indicated that GRP78 expression might be employed as a potential marker to indicate gastric carcinogenesis and subsequent progression, even prognosis.