Filamentous bacterial viruses. XIII. Molecular structure of the virion in projection.
Filamentous bacterial viruses. XIII. Molecular structure of the virion in projection.
复制标题
丝状细菌病毒。
DOI：
10.1016/0022-2836(74)90411-2
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发表时间：
1974
影响因子：
5.6
通讯作者：
D. Marvin
中科院分区：
文献类型：
--
作者：
E. Wachtel;R. Wiseman;W. Pigram;D. Marvin
丝状细菌病毒中电子密度的径向分布是根据 X 射线光纤衍射图的赤道强度计算得出的，分辨率为 15 Å。径向密度分布显示圆柱形分子，其中央高密度核心直径为 25 Å（解释为 DNA），周围环绕着内径 40 Å 和外径 60 Å 的壳（解释为蛋白质）。 I 类和 II 类病毒颗粒具有相似的径向密度分布。其他类型的模型被证明是不可能的。在干纤维中，病毒粒子的外围发生扭曲，使得病毒粒子能够堆积在晶格常数a≅ 55 Å的六方晶格中。圆柱模型通过重原子处理的纤维的衍射实验以及垂直于纤维轴的截面的电子显微镜证实。
The radial distribution of electron density in filamentous bacterial viruses is calculated from the equatorial intensity on X-ray fiber diffraction patterns, at a resolution of 15 Å. The radial density distribution shows a cylindrical molecule with a central high density core 25 Å in diameter (interpreted as DNA), surrounded by a shell with inner diameter 40 Å and outer diameter 60 Å (interpreted as protein). Both class I and class II virions have similar radial density distributions. Other types of model are shown to be unlikely. In dry fibers, the periphery of the virion is distorted to enable the virion to pack in a hexagonal lattice with lattice constant a≅ 55 A ̊. The cylindrical model is substantiated by diffraction experiments on fibers treated with heavy atoms, and by electron microscopy of sections taken perpendicular to the fiber axis.