miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer
miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer
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miR-150 作为与结直肠癌预后和治疗结果相关的潜在生物标志物
DOI：
10.1136/gutjnl-2011-301122
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发表时间：
2012-10-01
期刊：
影响因子：
24.5
通讯作者：
Qin, Huanlong
中科院分区：
文献类型：
--
作者：
Ma, Yanlei;Zhang, Peng;Qin, Huanlong
背景microRNA（miRNA）具有作为肿瘤预后生物标志物和治疗靶点的潜力。为探讨miRNA表达模式与结直肠癌（CRC）预后及治疗效果的关系，采用基因芯片技术检测肿瘤、腺瘤及正常结直肠组织中miRNA的表达谱，分析其在结直肠癌恶变过程中的表达。使用定量逆转录PCR（qRT-PCR）检测239例患者的肿瘤相关miRNA，并评估其与生存和辅助化疗反应的相关性。此外，为了验证研究结果，在185例CRC患者中，通过原位杂交（ISH）analysis.Results只有一个miR-150的肿瘤相关的miRNA与临床特征进行了分析，发现显示在三个组织组（正常，腺瘤和癌组织）中的表达水平降低，平行于增加大肠癌组织的癌变。在ISH和qRT-PCR分析中，与配对的非癌组织相比，肿瘤组织的miR-150表达水平降低，这表明miR-150表达水平与CRC相关。此外，肿瘤中miR-150低表达的患者生存期较短，对辅助化疗的反应较低，而肿瘤中miR-150高表达的患者对辅助化疗的反应较低。提示miR-150可作为结直肠癌预后和治疗效果的潜在生物标志物。
Background MicroRNAs (miRNA) have potential as prognostic biomarkers and therapeutic targets in cancer. A study was undertaken to investigate the association between miRNA expression patterns and the prognosis and therapeutic outcome of colorectal cancer (CRC).Methods miRNA expression profiling in tumour, adenoma and normal colorectal tissues was performed to identify tumour-related miRNAs in the course of colorectal malignant changes. Quantitative reverse transcription PCR (qRT-PCR) assays were used to measure tumour-related miRNA and to assess its association with survival and response to adjuvant chemotherapy in 239 patients. In addition, to validate the findings, associations of the tumour-related miRNA with clinical characteristics of CRC were analysed in 185 patients by in situ hybridisation (ISH) analysis.Results Only one miR-150 was found to show a decrease in expression levels in the three tissue groups (normal, adenoma and cancer tissue) in parallel with increasing carcinogenesis of the colorectal tissue. In both ISH and qRT-PCR analysis, tumour tissue had reduced levels of miR-150 expression compared with paired non-cancerous tissue, which indicated that the levels of miR-150 expression were associated with CRC. Moreover, patients whose tumours had low miR-150 expression had shorter survival and a worse response to adjuvant chemotherapy than patients whose tumours had high miRNA expression.Conclusions The miR-150 expression status of patients with CRC is associated with survival and response to adjuvant chemotherapy. It is suggested that miR-150 should be considered as a potential biomarker associated with the prognosis and therapeutic outcome in CRC.