Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance
Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance
复制标题
DOI：
10.1186/s12917-019-1836-6
复制
发表时间：
2019-04-08
影响因子：
2.6
通讯作者：
Chuma, Takehisa
中科院分区：
文献类型：
--
作者：
Vu Minh Duc;Nakamoto, Yuko;Chuma, Takehisa
本研究旨在调查日本鹿儿岛肉鸡分离的沙门氏菌的流行率、血清型和耐药性。2009~2012年，从日本鹿儿岛当地养殖场采集肉鸡192只，检出样品3071份。结果受检鸡场沙门氏菌阳性率为49.0%，盲肠样品3071份，检出沙门氏菌243株(7.9%)。所有沙门氏菌均为婴儿沙门氏菌(57.6%)、(140/243)、曼哈顿沙门氏菌(40.3%；98/243)和施瓦辛格伦沙门氏菌(2.1%；5/243)之一。婴儿沙门氏菌的比例从2009年的66.0%下降到2011年的50.0%，2012年上升到57.6%；曼哈顿沙门氏菌的比例从2009年的26.4%显著增加到2011年的50%，2012年温和下降到40.9%。大多数沙门氏菌对链霉素(95.1%)、磺胺甲恶唑(91.0%)和土霉素(91.4%)耐药。相比之下，所有沙门氏菌对氯霉素敏感。本研究与以往沙门氏菌药敏试验结果比较表明：沙门氏菌对氨苄西林(22.4%~55.1%)和头孢替辛(9.1%~52.7%)的耐药率显著增加。相比之下，在三个调查期内，氧氟沙星耐药菌株的百分比下降，从2004-2006年的20.8%下降到本研究期间(2009-2012年)的1.6%。此外，婴儿沙门氏菌对多种抗菌素表现出从敏感到对8种抗菌素的耐药性。113株(46.5%)沙门氏菌对6种以上抗菌药物多重耐药，其中以曼哈顿沙门氏菌为主。结论本次沙门氏菌的血清型和耐药谱与以往研究相比有明显变化。曼哈顿葡萄球菌的比例增加，对氨苄西林和头孢噻肟耐药的菌株比例也增加。
BackgroundThis study aimed to examine the prevalence, serovars, and antimicrobial resistance of Salmonella isolates from broiler chickens in Kagoshima, Japan. A total of 192 flocks and 3071 samples were collected from broiler chickens at local farms in Kagoshima, Japan from 2009 to 2012.ResultAmong the tested farms, 49.0% of flocks were positive for Salmonella, and 243 isolates were obtained from 3071 cecal samples (7.9%). All the Salmonella isolates were one of three serovars: S. Infantis (57.6%); (140/243), S. Manhattan (40.3%; 98/243 and S. Schwarzengrund (2.1%; 5/243). The proportion of S. Infantis isolates decreased from 66.0% in 2009 to 50.0% in 2011 but increased to 57.6% in 2012, while the proportion of S. Manhattan isolates significantly increased from 26.4 to 50% from 2009 to 2011, and decreased moderately to 40.9% in 2012. Most of the recovered Salmonella isolates were resistant to three antimicrobials, i.e., streptomycin (95.1%), sulfamethoxazole (91.0%) and oxytetracycline (91.4%). In contrast, all Salmonella strains were susceptible to chloramphenicol. Comparison of this study to previous studies of the antimicrobial susceptibility of Salmonella isolates showed that: the percentage of antibiotic-resistance isolates increased dramatically for two antibiotics, ampicillin (from 22.4 to 55.1%) and cefotaxime (from 9.1 to 52.7%). In contrast, the percentage of ofloxacin-resistant isolates decreased across the three survey periods, from 20.8% in 2004-2006 to 1.6% in the present study period (2009-2012). In addition, S. Infantis exhibited a variety of resistance to antimicrobials examined from sensitive to resistance to eight antimicrobials. Multidrug resistance to more than 6 six antimicrobials was detected in 113 (46.5%) of the isolates, and most of them were S. Manhattan.ConclusionsThere was a marked change in the serovars and antimicrobial resistance profiles of the Salmonella isolates in this study compared to those in previous studies. The percentage of S. Manhattan isolates increased as did the percentages of ampicillin- and cefotaxime-resistant isolates.