INTENSE COLD NOXIOUS-STIMULATION OF THE RAT HINDPAW INDUCES C-FOS EXPRESSION IN LUMBAR SPINAL-CORD NEURONS
INTENSE COLD NOXIOUS-STIMULATION OF THE RAT HINDPAW INDUCES C-FOS EXPRESSION IN LUMBAR SPINAL-CORD NEURONS
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DOI：
10.1016/0306-4522(94)90609-2
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发表时间：
1994-03-01
期刊：
影响因子：
3.3
通讯作者：
BESSON, JM
中科院分区：
文献类型：
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作者：
ABBADIE, C;HONORE, P;BESSON, JM
本研究评估了强冷刺激后，阿片类药物诱导的腰椎脊髓神经元fos样免疫反应性。在氨基甲酸乙酯麻醉下，将大鼠右脚置于15、10、0、-10、-15、-17.5或-20℃的调节温度浴中刺激，当脚掌温度在15至-10℃之间时，腰椎脊髓神经元无fos样免疫反应性或很少fos样免疫反应性（0-5个fos样免疫反应神经元/截面）。-15 ~ -20℃时，fos样免疫反应神经元数量随温度的降低而增加。在-20℃时，L3和L4节段、I-II层中fos样免疫反应神经元数量较多（约60个/切片），V-VI层中fos样免疫反应神经元数量较少（约20个）。III-IV层几乎不存在fos样免疫反应(
This study evaluated Fos-like immunoreactivity in lumbar spinal cord neurons following intense cold stimulation and then the modifications induced by opioid administration. Under urethane anaesthesia, the rat's right foot was stimulated by holding it in a regulated temperature bath at 15, 10, 0, -10, -15, -17.5 or -20 degrees C. There was no or little Fos-like immunoreactivity in lumbar spinal cord neurons when the paw was at temperatures between 15 and -10 degrees C (0-5 Fos-like immunoreactive neurons/section). The threshold to induce consistent c-Jos expression was -15 C. From -15 to -20 degrees C the number of Fos-like immunoreactive neurons increased with decreases in temperature. At -20 degrees C, Fos-like immunoreactive neurons were numerous in L3 and L4 segments, in laminae I-II(approximate to 60 Fos-like immunoreactive neurons/section) and to a lesser extent in laminae V-VI (approximate to 20). Almost no Fos-like immunoreactivity was present in laminae III-IV (