Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population.
Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population.
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DOI：
10.1093/jas/skaa235
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发表时间：
2020-07
影响因子：
3.3
通讯作者：
Qing Zhang;Jing Li;Tao Huang;Yifeng Zhang;Wenwu Xu;Lusheng Huang;H. Ai;Bin Yang
中科院分区：
文献类型：
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作者：
Qing Zhang;Jing Li;Tao Huang;Yifeng Zhang;Wenwu Xu;Lusheng Huang;H. Ai;Bin Yang
肺部疾病的发展，往往伴随着细菌感染，严重影响肉类生产和猪的福利。本研究调查了307头240日龄的猪，来自标准化饲养条件下饲养的8个品种的杂交猪，以研究肺病变程度、从支气管肺泡灌洗液的16 S rRNA测序推断的细菌负荷以及57个免疫细胞和25个血液学性状之间的关联。我们发现，所研究的猪遭受了大量和不同的肺部病变，支原体是最相关的细菌属。错误发现率为0.05（FDR<0.05），肺病变严重程度与较大的CD 8 +/CD 3+细胞比率、嗜中性粒细胞/淋巴细胞比率（NLR）和红细胞体积分布宽度标准差（RDW-SD）以及较低的CD 4-CD 8-/CD 3+显著相关，CD3+CD4-CD8-/PBMC（外周血单核细胞）和CD 14-CD 16-/PBMC细胞比率、平均红细胞血红蛋白浓度、淋巴细胞计数和淋巴细胞计数百分比，反映炎症状态，免疫抑制和缺氧的猪伴随着肺部病变的进展。支原体丰度与中性粒细胞计数、中性粒细胞计数百分比、NLR、单核细胞计数、红细胞体积分布宽度变异系数（RDW-CV）、RDW-SD呈正相关，与平均红细胞血红蛋白浓度、淋巴细胞计数、淋巴细胞计数百分比呈负相关，这些相关性与肺部病变基本一致，支持肺部病变和支原体感染的共病。我们还观察到非线性相关性，即中性粒细胞计数和中性粒细胞计数百分比的急剧增加仅发生在支原体丰度高于人群平均水平时。本研究结果有助于了解猪支原体相关性肺病时宿主免疫状态的变化。
Developments of pulmonary diseases, often accompanied by infections of bacteria, severely affect meat production and welfare of pigs. This study investigated 307 pigs at age of 240 days from an eight-breed cross reared under standardized housing conditions for associations among extent of lung lesions, bacteria load inferred from 16S rRNA sequencing of bronchoalveolar lavage fluid, as well as 57 immune cells and 25 hematological traits. We showed that the pigs under study suffered substantial and varied lung lesions, and the Mycoplasma is the most associated bacteria genera. At a false discovery rate of 0.05 (FDR<0.05), severity of lung lesions were significantly associated with greater CD8+ to CD3+ cell ratio, neutrophil-to-lymphocyte ratio (NLR), and standard deviation of red blood cell volume distribution width (RDW-SD), and lower CD4-CD8-/CD3+, CD3+CD4-CD8-/PBMCs (peripheral blood mononuclear cells) and CD14-CD16-/PBMCs cell ratios, mean corpuscular hemoglobin concentration, lymphocyte count and lymphocyte count percentage, reflecting an status of inflammation, immune suppression and hypoxia of the pigs accompanying the progression of lung lesion. The Mycoplasma abundance showed positive correlations with neutrophil count, neutrophil count percentage, NLR, monocyte count , coefficient of variation in red blood cell volume distribution width (RDW-CV), and RDW-SD, and negative correlations with mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage, these correlations are largely consistent with those of lung lesions, supporting the comorbidity of lung lesions and Mycoplasma infection. We also observed nonlinear associations that sharp increases in neutrophil count and neutrophil count percentage occurred only when Mycoplasma abundance raised above the population-average level. The results provide helpful insights into the changes of host immune status in response to Mycoplasma relevant lung diseases in pigs.