Promoter-proximal CCCTC-factor binding is associated with an increase in the transcriptional pausing index.
Promoter-proximal CCCTC-factor binding is associated with an increase in the transcriptional pausing index.
复制标题
启动子近端 CCCTC 因子结合与转录暂停指数的增加相关。
DOI：
10.1093/bioinformatics/bts596
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sethupathy,Praveen
中科院分区：
文献类型：
--
作者：
Paredes,SurHerrera;Melgar,MichaelF;Sethupathy,Praveen
动机：二十多年来，人们已经知道，RNA聚合酶II（RNAPII）启动转录后，它可以在生产性延伸之前立即进入转录起始位点下游的暂停或停滞状态。高通量基因组技术的最新进展促进了RNAPII在启动子处暂停是一种普遍的生理调节现象的发现。暂停的分子基础尚未完全理解。CCCTC因子（CTCF）是一种普遍存在的核因子，具有多种调节功能，包括最近发现的促进RNAPII暂停在剪接sites.Results的作用：在这项研究中，我们分析了CTCF结合位点和新生的转录组数据从三种不同的细胞类型，并发现启动子近端CTCF结合显着相关RNAPII暂停。联系：praveen_sethupathy@med.unc. edu补充信息：补充数据可在Bioinformaticsonline。
Motivation:It has been known for more than 2 decades that after RNA polymerase II (RNAPII) initiates transcription, it can enter into a paused or stalled state immediately downstream of the transcription start site before productive elongation. Recent advances in high-throughput genomic technologies facilitated the discovery that RNAPII pausing at promoters is a widespread physiologically regulated phenomenon. The molecular underpinnings of pausing are incompletely understood. The CCCTC-factor (CTCF) is a ubiquitous nuclear factor that has diverse regulatory functions, including a recently discovered role in promoting RNAPII pausing at splice sites.Results:In this study, we analyzed CTCF binding sites and nascent transcriptomic data from three different cell types, and found that promoter-proximal CTCF binding is significantly associated with RNAPII pausing.Contact:praveen_sethupathy@med.unc.eduSupplementary information:Supplementary data are available atBioinformaticsonline.